Rabbit Anti-PLEKHA1 Recombinant Antibody (clone R01-4F5)
CAT#: VS3-FY1894
This anti-PLEKHA1 antibody detected positive in western blot analysis of TAPP1 in K562 lysates. The recombinant monoclonal antibody exhibits a specific binding affinity towards human PLEKHA1. The antibody is recommended for detection of PLEKHA1 protein by WB, IP.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2043/35_B5_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2043/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2043/18020_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2043/18020_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2043/18020_A_8_4.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Pancreatic endocrine cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2043/18020_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2043/18020_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2043/18020_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2043/18020_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107679-PLEKHA1/subcellular
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❯
WB
Figure 1 Anti-PLEKHA1 Antibody (VS3-FY1894) in WB.
The TAPP1 expression in K562 lysates were detected using the anti-PLEKHA1 by western blot assay.
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❯
Specifications
- Immunogen
- Synthetic peptide of human TAPP-1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PLEKHA1
- Species Reactivity
- Human
- Clone
- R01-4F5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 46 kDa, while the observed molecular weight stands at 46 kDa.
- Related Disease
- Macular Degeneration, Age-Related, 1 and Childhood Kidney Cell Carcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- TAPP1
- Gene ID
- 59338
- UniProt ID
- Q9HB21
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus
- Protein Refseq
- NP_001001974.1; NP_001182537.1; NP_001317107.1
- Function
- Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PLEKHA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2747 | Hi-Affi™ Recombinant Rabbit Anti-PLEKHA1 Monoclonal Antibody (DS2747AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS4-CJ78 | Recombinant Rabbit Anti-PLEKHA1 Antibody (clone 16C9) | ELISA, WB | Rabbit IgG |
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