Rabbit Anti-PMS2 Recombinant Antibody (clone R06-5G3)
CAT#: VS3-FY1626
This anti-PMS2 antibody can be functioned in western blot analysis of PMS2 in Jurkat, Hela lysates. The recombinant monoclonal antibody has been developed to target human PMS2. It is suitable for use in a variety of assays, including WB, IP.
Tested Data
WB
Figure 1 Anti-PMS2 Antibody (VS3-FY1626) in WB.
The PMS2 expression in Jurkat, Hela lysates were detected using the anti-PMS2 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PMS2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PMS2
- Species Reactivity
- Human
- Clone
- R06-5G3
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 96 kDa, while the observed molecular weight stands at 96 kDa.
- Related Disease
- Lynch Syndrome 4 and Mismatch Repair Cancer Syndrome 4
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- MLH4; PMSL2; HNPCC4; PMS2CL
- Gene ID
- 5395
- UniProt ID
- P54278
- Sequence Similarities
- Belongs to the DNA mismatch repair MutL/HexB family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_000526.2; NP_001308932.1; NP_001308933.1; NP_001308934.1; NP_001308935.1
- Function
- Component of the post-replicative DNA mismatch repair system (MMR).
Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Possesses an ATPase activity, but in the absence of gross structural changes, ATP hydrolysis may not be necessary for proficient mismatch repair.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PMS2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1832z | Mouse Anti-PMS2 Recombinant Antibody (clone 5G11) | WB, ELISA, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1291CT | Recombinant Mouse anti-Human PMS2 Monoclonal antibody (274D2362) | IHC-P, WB |
| CAT | Product Name | Application | Type |
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| MOR-2765 | Hi-Affi™ Recombinant Rabbit Anti-PMS2 Monoclonal Antibody (DS2765AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1251-CN | Recombinant Rabbit Anti-PMS2 Monoclonal Antibody (SY08-09) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK799 | Rabbit Anti-PMS2 Recombinant Antibody (clone SY08-09) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1158 | Recombinant Mouse Anti-PMS2 Antibody (clone 6G12E1) | ELISA, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5520 | Anti-PMS2 Immunohistochemistry Kit | IHC |
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