Rabbit Anti-PMS2 Recombinant Antibody (clone R06-5G3) (CAT#: VS3-FY1626)

• Immunogen
• Synthetic peptide of human PMS2

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human PMS2

• Species Reactivity
• Human

• Clone
• R06-5G3

• Applications
• Western Blot, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 96 kDa, while the observed molecular weight stands at 96 kDa.

• Related Disease
• Lynch Syndrome 4 and Mismatch Repair Cancer Syndrome 4

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunoprecipitation: 1/20

• Alternative Names
• MLH4; PMSL2; HNPCC4; PMS2CL

• Gene ID
• 5395

• UniProt ID
• P54278

• Sequence Similarities
• Belongs to the DNA mismatch repair MutL/HexB family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphoprotein

• Protein Refseq
• NP_000526.2; NP_001308932.1; NP_001308933.1; NP_001308934.1; NP_001308935.1

• Function
• Component of the post-replicative DNA mismatch repair system (MMR).
  Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Possesses an ATPase activity, but in the absence of gross structural changes, ATP hydrolysis may not be necessary for proficient mismatch repair.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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