Rabbit Anti-POLR2E Recombinant Antibody (clone R05-8J5)
CAT#: VS3-FY1839
This R05-8J5 recombinant monoclonal antibody is specific for human POLR2E. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The binding specificity of the RNA polymerases I/II/III subunit ABC1 antibody was determined using western blot analysis of POLR2E in K562, C6, 3T3, Hela lysates.
Tested Data
WB
Figure 1 Anti-POLR2E Antibody (VS3-FY1839) in WB.
The POLR2E expression in K562, C6, 3T3, Hela lysates were detected using the anti-RNA Polymerases I/II/III Subunit ABC1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human POLR2E
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat POLR2E
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-8J5
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 25 kDa, while the observed molecular weight stands at 25 kDa.
- Related Disease
- Esophageal Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- RPB5; XAP4; RPABC1; hRPB25; hsRPB5
- Gene ID
- 5434
- UniProt ID
- P19388
- Sequence Similarities
- Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.
- Cellular Localization
- DNA-directed RNA polymerase, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys115 (NX_P19388 [NX_P19388-1])
Modification sites at PhosphoSitePlus (P19388)
Glycosylation from GlyGen (P19388) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011526372.1; XP_016882372.1; XP_054177215.1; XP_054177216.1; NP_001303252.1
- Function
- DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "POLR2E"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2784 | Hi-Affi™ Recombinant Rabbit Anti-POLR2E Monoclonal Antibody (DS2784AB) | IHC-P, WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY400 | Mouse Anti-NHP POLR2E Recombinant Antibody (clone OTI3B5) | WB, IHC, IF, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5556 | Anti-POLR2E Immunohistochemistry Kit | IHC |
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