Mouse Anti-POMC Recombinant Antibody (clone 6D2B5)
CAT#: VS7-0425-WR649
This product is a mouse IgG1 antibody that binds to human POMC. The antibody 6D2B5 recognizes specific amino acid domain of human POMC (AA: 1-150) . It can be used for ICC assay.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human pituitary gland
Immunohistochemical staining shows high expression in pituitary gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/46135/849_266_200x200.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46135/146483_A_8_4.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46135/146483_A_6_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (HMC-1, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115138-POMC/subcellular
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Specifications
- Immunogen
- Recombinant human POMC (AA: 1-150) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human POMC
- Species Reactivity
- Human
- Clone
- 6D2B5
- Molecule Class
- Prohormone Polypeptide Precursor
- Applications
- ICC
- Conjugate
- Unconjugated
- Related Disease
- Obesity (OBESITY); Obesity, early-onset, with adrenal insufficiency and red hair (OBAIRH)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- POMC; Corticotropin-Like Intermediary Peptide; Gamma-MSH; ACTH; CLIP; Melanotropin Beta; Melanotropin Gamma; Beta-Endorphin; Beta-LPH; Adrenocorticotropin; MSH; POC; Proopiomelanocortin Preproprotein; Beta-Melanocyte-Stimulating Hormone;
- Gene ID
- 5443
- UniProt ID
- P01189
- Long Name
- Proopiomelanocortin
- Cellular Localization
- Secreted
- Post Translation Modifications
- Acetylation; Amidation; Cleavage on pair of basic residues; Disulfide bond; Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_000930.1
- Function
- Endorphin; Hormone
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0426MZ | Chicken Anti-POMC Polyclonal IgY | IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4526 | Hi-Affi™ Recombinant Rabbit Anti-POMC Monoclonal Antibody (TH34DS) | WB | IgG |
| MOR-4527 | Hi-Affi™ Recombinant Rabbit Anti-POMC Monoclonal Antibody (TH35DS) | WB, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0017J | Mouse Anti-POMC Recombinant Antibody (ZG-0017J) | IHC-P, IF | Mouse IgG |
| VS1-YJ4 | Mouse Anti-POMC Recombinant Antibody (clone 57) | ELISA, IHC-P | Mouse IgG1 |
| VS1-YJ5 | Mouse Anti-POMC Recombinant Antibody (clone 57), HRP | IHC-P | Mouse IgG1 |
| VS3-FY1165 | Recombinant Mouse Anti-POMC Antibody (clone 6D4-7B1-1A1) | IHC-P | Mouse IgG1 |
| VS7-0425-WR648 | Mouse Anti-POMC Recombinant Antibody (clone 5D3A10) | WB, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5562 | Anti-POMC Immunohistochemistry Kit | IHC | |
| VS-0525-XY5563 | Anti-Mouse POMC Immunohistochemistry Kit | IHC | |
| VS-0525-XY5564 | Anti-Rat POMC Immunohistochemistry Kit | IHC |
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