Rabbit Anti-PON2 Recombinant Antibody (clone R05-1J3)
CAT#: VS3-FY1841
This rabbit recombinant monoclonal antibody shows positive application of western blot analysis of PON2 in K562, Hela lysates. It has been developed to target human PON2. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This R05-1J3 antibody is validated for western blot analysis of PON2 in K562, Hela lysates.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IHC staining of human liver
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25430/54119_A_8_4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25430/54119_B_9_5.jpg
Normal Tissue
Figure 3 Adrenal gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25430/54119_B_6_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25430/54119_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25430/54119_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25430/54119_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25430/54119_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25430/54119_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105854-PON2/subcellular
❮
❯
WB
Figure 1 Anti-PON2 Antibody (VS3-FY1841) in WB.
The PON2 expression in K562, Hela lysates were detected using the anti-PON2 by western blot assay.
❮
❯
Specifications
- Immunogen
- Recombinant human PON2 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PON2
- Species Reactivity
- Human
- Clone
- R05-1J3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 40-42 kDa.
- Related Disease
- Vascular diseases and many quantitative phenotypes related to diabetes
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- PON2; paraoxonase 2
- Gene ID
- 5445
- UniProt ID
- Q15165
- Sequence Similarities
- Belongs to the paraoxonase family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- The signal sequence is not cleaved. (Q15165-PON2_HUMAN)
Glycosylation at Asn254, Asn269, and Asn323 (NX_Q15165 [NX_Q15165-2])
Modification sites at PhosphoSitePlus (Q15165)
Glycosylation from GlyConnect PON2_HUMAN (1741)
Glycosylation from GlyGen (Q15165) 5 sites, 9 N-linked glycans (2 sites), 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005250510.1; XP_016867846.1; XP_054214449.1; XP_054214450.1; XP_054214451.1
- Function
- Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PON2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2412z | Mouse Anti-PON2 Recombinant Antibody (clone 29B9) | WB, ELISA, IF, IHC, IP | Mouse IgG2b, κ |
| MOB-1471CT | Recombinant Mouse anti-Human PON2 Monoclonal antibody (BG4F7) | IP, WB | |
| MRO-1255-CN | Recombinant Rabbit Anti-PON2 Monoclonal Antibody (JG35-80) | WB, IHC, IP | Rabbit IgG |
| VS3-WK455 | Rabbit Anti-PON2 Recombinant Antibody (clone JG35-80) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2789 | Hi-Affi™ Recombinant Rabbit Anti-PON2 Monoclonal Antibody (DS2789AB) | WB, IHC-P, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1701 | Anti-PON2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5570 | Anti-Human PON2 Immunohistochemistry Kit | IHC |
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