Rabbit Anti-PON2 Recombinant Antibody (clone R05-1J3) (CAT#: VS3-FY1841)

This rabbit recombinant monoclonal antibody shows positive application of western blot analysis of PON2 in K562, Hela lysates. It has been developed to target human PON2. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This R05-1J3 antibody is validated for western blot analysis of PON2 in K562, Hela lysates.


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WB

Figure 1 Anti-PON2 Antibody (VS3-FY1841) in WB.

Figure 1 Anti-PON2 Antibody (VS3-FY1841) in WB.

The PON2 expression in K562, Hela lysates were detected using the anti-PON2 by western blot assay.


Specifications

  • Immunogen
  • Recombinant human PON2 protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human PON2
  • Species Reactivity
  • Human
  • Clone
  • R05-1J3
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 40-42 kDa.
  • Related Disease
  • Vascular diseases and many quantitative phenotypes related to diabetes

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • PON2; paraoxonase 2
  • Sequence Similarities
  • Belongs to the paraoxonase family.
  • Cellular Localization
  • Membrane
  • Post Translation Modifications
  • The signal sequence is not cleaved. (Q15165-PON2_HUMAN)
    Glycosylation at Asn254, Asn269, and Asn323 (NX_Q15165 [NX_Q15165-2])
    Modification sites at PhosphoSitePlus (Q15165)
    Glycosylation from GlyConnect PON2_HUMAN (1741)
    Glycosylation from GlyGen (Q15165) 5 sites, 9 N-linked glycans (2 sites), 1 O-linked glycan (1 site)
  • Function
  • Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "PON2"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2789 Hi-Affi™ Recombinant Rabbit Anti-PON2 Monoclonal Antibody (DS2789AB) WB, IHC-P, IP IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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