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    AbPlus™ Anti-PPIE Magnetic Beads (VS-0724-YC191) (CAT#: VS-0724-YC191)

    The AbPlus Anti-PPIE Magnetic Beads (VS-0724-YC191) is an innovative affinity resin which is bound with anti-PPIE specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of PPIE protein under native and denaturing conditions.

    Specific Inquiry
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19357/1617_E5_1_selected.jpg
    Figure 2 IHC staining of human cerebellum
    Figure 2 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong nuclear positivity.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Glial cells
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Glandular cells
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_8_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_8_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Nuclear
    Cells in tubules
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Leydig cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_6_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: Not detected
    Intensity: Weak
    Quantity: <25%
    Location: Nuclear
    Non-germinal center cells
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_9_8.jpg
    Figure 9 Heart
    Figure 9 Heart
    (Cardiomyocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_B_5_6.jpg
    RNA Expression
    Figure 10 RNA cell line category: Low cell line specificity
    Figure 10 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000084072-PPIE/subcellular

    Specifications

    • Applications
    • Immunoprecipitation, Protein Purification
    • Matrix
    • Magnetic bead
    • Bead Ligand
    • Anti-PPIE specific antibody
    • Target
    • PPIE
    • Immunogen
    • Synthetic peptide corresponding to the N-terminus of the human PPIE.
    • Target Species
    • Human, Mouse
    • Bead Capacity
    • 40 mg/mL
    • Bead size
    • 25 μm
    • Format
    • Suspension
    • Concentration
    • 2 mg/mL
    • Buffer
    • PBS, pH 7.4
    • Preservative
    • 0.1% Sodium azide
    • Storage
    • Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.

    Applications

    • Application Notes
    • The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

    Target

    • Introduction
    • The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity.
    • Alternative Names
    • CypE; CYP33; CYP-33

    Downloads

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    CAT Product Name Application Type
    MOB-0120CT Recombinant Mouse anti-Human PPIE Monoclonal antibody (EML2321) WB

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