Recombinant Mouse anti-Human PPIE Monoclonal antibody (EML2321) (CAT#: MOB-0120CT)
Recombinant Mouse monoclonal antibody to Human PPIE, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19357/1617_E5_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19357/1617_E5_1_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_B_9_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_8_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_8_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_8_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_8_5.jpg
(Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_6_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_9_8.jpg
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_A_9_8.jpg
Figure 9 Heart
(Cardiomyocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_B_5_6.jpg
(Cardiomyocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20131/47928_B_5_6.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000084072-PPIE/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000084072-PPIE/subcellular
Specifications
- Immunogen
- Recombinant fragment: ATTKRVLYVG GLAEEVDDKV LHAAFIPFGD ITDIQIPLDY ETEKHRGFAF VEFELAEDAA AAIDNMNESE LFGRTIRVNL AKPMRIKEGS SRPVWSDDD, corresponding to amino acids 2-101 of Human Cyclophilin E
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML2321
- Applications
- Used for immunoassay techniques such as: Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- PPIE; peptidylprolyl isomerase E (cyclophilin E); CYP33; CYP-33; peptidyl-prolyl cis-trans isomerase E; PPIase E; rotamase E; cyclophilin-33;
- Gene ID
- 10450
- UniProt ID
- Q9UNP9
- Protein Refseq
- NP_001181936
- Function
- RNA binding; RNA binding; cyclosporin A binding; isomerase activity; nucleotide binding; peptidyl-prolyl cis-trans isomerase activity; peptidyl-prolyl cis-trans isomerase activity; protein binding;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0433MZ | Chicken Anti-PPIE Polyclonal IgY | WB | Chicken antibody |
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