Mouse Anti-PPIH Recombinant Antibody (clone 1C6)
CAT#: VS-0625-FY65
The Mouse IgG1 monoclonal antibody, clone 1C6, targets PPIH in Human, Mouse, and Rat. Its immunogen is full-length recombinant human PPIH protein, with an epitope spanning amino acids 1-90. With an approximate antigen size of 19 kDa, this antibody is validated for Western Blot at a dilution of 1:2000, providing reliable detection for PPIH.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59019/ihc_selected.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59019/131423_A_8_3.jpg
Normal Tissue
Figure 3 Smooth muscle
Smooth muscle cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59019/131423_B_5_7.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59019/131423_A_7_8.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171960-PPIH/subcellular
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Specifications
- Immunogen
- A full-length recombinant human PPIH protein (NP_006338) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 1C6
- Applications
- Western Blot
- Related Disease
- Gastric Tubular Adenocarcinoma and Myxosarcoma
Product Property
- Purification
- Purified with Protein A or G affinity chromatography
- Purity
- >90% as determined by SDS-PAGE
- Molecular Weight
- The predicted antigen size is approximately 19 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.3, 1% BSA, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:2000
Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- CYPH; CYP-20; USA-CYP; SnuCyp-20
- Gene ID
- 10465
- UniProt ID
- O43447
- Long Name
- peptidylprolyl isomerase H
- Sequence Similarities
- Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.
- Cellular Localization
- Nucleus speckle {ECO:0000269 PubMed:9570313}
Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys61 and Lys101
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan
- Protein Refseq
- XP_005270419.1; XP_047286822.1; XP_054189823.1; XP_054189824.1; NP_001317439.1
- Function
- PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357).
Participates in pre-mRNA splicing.
May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
May act as a chaperone.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PPIH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0435MZ | Chicken Anti-PPIH Polyclonal IgY | WB | Chicken antibody |
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