Rabbit Anti-PRDX4 Recombinant Antibody (clone R09-7F9) (CAT#: VS3-FY1827)

This R09-7F9 recombinant monoclonal antibody has the ability to specifically bind to human PRDX4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. This peroxiredoxin 4 antibody was used in western blot analysis of peroxiredoxin 4 in 293T lysates.

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WB

Figure 1 Anti-PRDX4 Antibody (VS3-FY1827) in WB.

Figure 1 Anti-PRDX4 Antibody (VS3-FY1827) in WB.

The Peroxiredoxin 4 expression in 293T lysates were detected using the anti-Peroxiredoxin 4 by western blot assay.


Specifications

  • Immunogen
  • Synthetic peptide of human peroxiredoxin 4
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat PRDX4
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R09-7F9
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin
  • MW
  • The calculated weight of the target protein is approximately 31 kDa, while the observed molecular weight stands at 27 kDa.
  • Related Disease
  • Hypoascorbemia and Filariasis

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100

Target

  • Alternative Names
  • PRDX4; AOE37-2; AOE372; HEL-S-97n; PRX-4; peroxiredoxin 4
  • Sequence Similarities
  • Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
  • Cellular Localization
  • Cytoplasm, Endoplasmic reticulum
  • Post Translation Modifications
  • The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond. (Q13162-PRDX4_HUMAN)
    Modification sites at PhosphoSitePlus (Q13162)
    Modification sites at neXtProt (NX_Q13162)
    Glycosylation from GlyGen (Q13162) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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