Rabbit Anti-PRDX4 Recombinant Antibody (clone R09-7F9)

CAT#: VS3-FY1827

This R09-7F9 recombinant monoclonal antibody has the ability to specifically bind to human PRDX4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. This peroxiredoxin 4 antibody was used in western blot analysis of peroxiredoxin 4 in 293T lysates.

Tested Data
Figure 1 Anti-PRDX4 Antibody (VS3-FY1827) in WB.

Specifications

  • Immunogen
  • Synthetic peptide of human peroxiredoxin 4
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat PRDX4
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R09-7F9
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin
  • MW
  • The calculated weight of the target protein is approximately 31 kDa, while the observed molecular weight stands at 27 kDa.
  • Related Disease
  • Hypoascorbemia and Filariasis

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100

Target

  • Alternative Names
  • PRDX4; AOE37-2; AOE372; HEL-S-97n; PRX-4; peroxiredoxin 4
  • Sequence Similarities
  • Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
  • Cellular Localization
  • Cytoplasm, Endoplasmic reticulum
  • Post Translation Modifications
  • The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond. (Q13162-PRDX4_HUMAN)
    Modification sites at PhosphoSitePlus (Q13162)
    Modification sites at neXtProt (NX_Q13162)
    Glycosylation from GlyGen (Q13162) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Certificate of Analysis Lookup

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Isotype Control

Secondary Antibody

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