Rabbit Anti-PRDX6 Recombinant Antibody (clone R03-7F5) (CAT#: VS3-FY2062)
This rabbit recombinant monoclonal antibody has been developed to bind specifically to human PRDX6. The antibody is purified by affinity chromatography. It is suitable for use in a variety of assays, including WB, IP. This R03-7F5 antibody is validated for western blot analysis of peroxiredoxin 6 in A549, HL-60, U2OS lysates.
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WB
Figure 1 Anti-PRDX6 Antibody (VS3-FY2062) in WB.
The Peroxiredoxin 6 expression in A549, HL-60, U2OS lysates were detected using the anti-Peroxiredoxin 6 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human peroxiredoxin 6
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRDX6
- Species Reactivity
- Human
- Clone
- R03-7F5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 25 kDa, while the observed molecular weight stands at 25 kDa.
- Related Disease
- Gnathomiasis and Down Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; LPCAT-5; HEL-S-128m
- Gene ID
- 9588
- UniProt ID
- P30041
- Sequence Similarities
- Belongs to the peroxiredoxin family. Prx6 subfamily.
- Cellular Localization
- Cytoplasm, Lysosome
- Post Translation Modifications
- Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress. (P30041-PRDX6_HUMAN)
Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme ().
The phosphorylated form exhibits a greater lysophosphatidylcholine acyltransferase activity compared to the non-phosphorylated form (PubMed:26830860).
(P30041-PRDX6_HUMAN)
Ubiquitination at Lys209 (NX_P30041 [NX_P30041-1])
Modification sites at PhosphoSitePlus (P30041)
Glycosylation from GlyGen (P30041) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_004896.1
- Function
- Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis.
Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "PRDX6"
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2153z | Mouse Anti-PRDX6 Recombinant Antibody (clone 28D1) | WB, ELISA, FC, ICC, IF | Mouse IgG2a |
| MOB-1403CT | Recombinant Mouse anti-Human PRDX6 Monoclonal antibody (2B22) | ELISA, IP, WB | |
| MRO-1263-CN | Recombinant Mouse Anti-PRDX6 Monoclonal Antibody (7G1) | WB, IHC, IF, FC | Mouse IgG1 |
| MRO-1264-CN | Recombinant Rabbit Anti-PRDX6 Monoclonal Antibody (JM11-13) | WB, IP, IF | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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