Rabbit Anti-PRDX6 Recombinant Antibody (clone R03-7F5)
CAT#: VS3-FY2062
This rabbit recombinant monoclonal antibody has been developed to bind specifically to human PRDX6. The antibody is purified by affinity chromatography. It is suitable for use in a variety of assays, including WB, IP. This R03-7F5 antibody is validated for western blot analysis of peroxiredoxin 6 in A549, HL-60, U2OS lysates.
Tested Data
WB
Figure 1 Anti-PRDX6 Antibody (VS3-FY2062) in WB.
The Peroxiredoxin 6 expression in A549, HL-60, U2OS lysates were detected using the anti-Peroxiredoxin 6 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human peroxiredoxin 6
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRDX6
- Species Reactivity
- Human
- Clone
- R03-7F5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 25 kDa, while the observed molecular weight stands at 25 kDa.
- Related Disease
- Gnathomiasis and Down Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; LPCAT-5; HEL-S-128m
- Gene ID
- 9588
- UniProt ID
- P30041
- Sequence Similarities
- Belongs to the peroxiredoxin family. Prx6 subfamily.
- Cellular Localization
- Cytoplasm, Lysosome
- Post Translation Modifications
- Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress. (P30041-PRDX6_HUMAN)
Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme ().
The phosphorylated form exhibits a greater lysophosphatidylcholine acyltransferase activity compared to the non-phosphorylated form (PubMed:26830860).
(P30041-PRDX6_HUMAN)
Ubiquitination at Lys209 (NX_P30041 [NX_P30041-1])
Modification sites at PhosphoSitePlus (P30041)
Glycosylation from GlyGen (P30041) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_004896.1
- Function
- Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis.
Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRDX6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2153z | Mouse Anti-PRDX6 Recombinant Antibody (clone 28D1) | WB, ELISA, FC, ICC, IF | Mouse IgG2a |
| MOB-1403CT | Recombinant Mouse anti-Human PRDX6 Monoclonal antibody (2B22) | ELISA, IP, WB | |
| MRO-1263-CN | Recombinant Mouse Anti-PRDX6 Monoclonal Antibody (7G1) | WB, IHC, IF, FC | Mouse IgG1 |
| MRO-1264-CN | Recombinant Rabbit Anti-PRDX6 Monoclonal Antibody (JM11-13) | WB, IP, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2841 | Hi-Affi™ Recombinant Rabbit Anti-PRDX6 Monoclonal Antibody (DS2841AB) | WB, IP, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1401 | AbPlus™ Anti-PRDX6 Magnetic Beads (VS-0724-YC1401) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1737 | Anti-PRDX6 Immunohistochemistry Kit | IHC |
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