Rabbit Anti-PREPL Recombinant Antibody (clone R08-8A2)
CAT#: VS3-FY2067
This R08-8A2 recombinant monoclonal antibody has the ability to specifically bind to human PREPL. It cross-reacts with mouse, rat, hamster. It is suitable for use in a variety of assays, including WB, IHC-P, IP. The PPCEL antibody is recommended for detection of target protein by western blot analysis of PPCEL/PREPL in mouse brain lysates and western blot analysis of PPCEL/PREPL in Hela, CHO-K1, C6 lysates.
Tested Data
WB
Figure 1 Anti-PREPL Antibody (VS3-FY2067) in WB.
The PPCEL/PREPL expression in mouse brain lysates were detected using the anti-PPCEL by western blot assay.
WB
Figure 2 Anti-PREPL Antibody (VS3-FY2067) in WB.
The PPCEL/PREPL expression in Hela, CHO-K1, C6 lysates were detected using the anti-PPCEL/PREPL by western blot assay.
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Specifications
- Immunogen
- Recombinant human PPCEL/PREPL protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster PREPL
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R08-8A2
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 84 kDa, while the observed molecular weight stands at 72 kDa.
- Related Disease
- Myasthenic Syndrome, Congenital, 22 and Cystinuria
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- CMS22
- Gene ID
- 9581
- UniProt ID
- Q4J6C6
- Sequence Similarities
- Belongs to the peptidase S9A family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Golgi apparatus, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys426 and Lys439 (NX_Q4J6C6 [NX_Q4J6C6-1])
Modification sites at PhosphoSitePlus (Q4J6C6)
Glycosylation from GlyGen (Q4J6C6) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016860874.1; XP_047302397.1; XP_047302398.1; XP_047302399.1; XP_047302400.1
- Function
- Regulates trans-Golgi network morphology and sorting by regulating the membrane binding of the AP-1 complex.
May play a role in the regulation of synaptic vesicle exocytosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PREPL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5664 | Anti-PREPL Immunohistochemistry Kit | IHC | |
| VS-0525-XY5665 | Anti-Mouse PREPL Immunohistochemistry Kit | IHC |
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