Rabbit Anti-PRKCD Recombinant Antibody (clone R20-F), FITC
CAT#: ZG-0416U
Rabbit anti-PRKCD antibody (clone R20-F) was conjugated with FITC under optimal conditions. The FITC-conjugated antibody product displayed high specificity against the PRKCD protein of Human. And the solution is free of unconjugated FITC and unconjugated antibody.
Tested Data
Gene Expression
FC
Figure 1 Rabbit Anti-PRKCD Antibody (ZG-0416U) in FC
Profile of PKCδ expression in peripheral blood lymphocytes analyzed by the BD FACSCanto II. Blood cells of healthy patient were fixed, permeabilized and stained with anti-PKCδ FITC (blue, 10 μl per test) or with an isotype control (black).
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1863/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1890/if_selected.jpg
Subcellular Location
Figure 3 Cerebral cortex
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_8_3.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_6_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (SK-BR-3, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163932-PRKCD
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRKCD
- Species Reactivity
- Human
- Clone
- R20-F
- Applications
- FC
- Conjugate
- FITC
- Related Disease
- Autoimmune Lymphoproliferative Syndrome, Type Iii and Autoimmune Lymphoproliferative Syndrome.
Product Property
- Buffer
- Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C, in the dark. Do not freeze.
Applications
- Application Notes
- This antibody has been used 10 µL per test for Flow Cytometry. Specific conditions should be optimized by the end user.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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