Rabbit Anti-PRKRA Recombinant Antibody (clone R04-8F7)
CAT#: VS3-FY1813
This R04-8F7 recombinant monoclonal antibody has the ability to specifically bind to human PRKRA. It cross-reacts with mouse, rat. The specificity of the PRKRA antibody was confirmed by western blot analysis of PABPN1 in Jurkat, Hela, COS7, Raji lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
Tested Data
Gene Expression
WB
Figure 1 Anti-PRKRA Antibody (VS3-FY1813) in WB.
The PABPN1 expression in Jurkat, Hela, COS7, Raji lysates were detected using the anti-PABPN1 by western blot assay.
IHC
Figure 2 Anti-PRKRA Antibody (VS3-FY1813) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-PABPN1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34996/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34996/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34997/135066_B_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34997/135066_A_5_6.jpg
Normal Tissue
Figure 5 Epididymis
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34997/135066_A_2_6.jpg
RNA Expression
Figure 6 RNA cell line categoryi : Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180228-PRKRA/subcellular
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Specifications
- Immunogen
- Synthetic peptide of human PACT (phospho-KR activating protein)/PRKRA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PRKRA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-8F7
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 34 kDa, while the observed molecular weight stands at 34 kDa.
- Related Disease
- Human breast cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- RAX; PACT; DYT16; HSD14
- Gene ID
- 8575
- UniProt ID
- O75569
- Sequence Similarities
- Belongs to the PRKRA family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells.
Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287.
Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.
(O75569-PRKRA_HUMAN)
Modification sites at PhosphoSitePlus (O75569)
Modification sites at neXtProt (NX_O75569)
Glycosylation from GlyGen (O75569) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011510365.1; XP_047302094.1; XP_054200276.1; XP_054200277.1; XP_054200278.1
- Function
- Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRKRA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1528CT | Recombinant Mouse anti-Human PRKRA Monoclonal antibody (EML878) | IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2864 | Hi-Affi™ Recombinant Rabbit Anti-PRKRA Monoclonal Antibody (DS2864AB) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1759 | Anti-PRKRA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5711 | Anti-Mouse PRKRA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5710 | Anti-Human PRKRA Immunohistochemistry Kit | IHC |
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