Rabbit Anti-PRKRA Recombinant Antibody (clone R04-8F7) (CAT#: VS3-FY1813)
This R04-8F7 recombinant monoclonal antibody has the ability to specifically bind to human PRKRA. It cross-reacts with mouse, rat. The specificity of the PRKRA antibody was confirmed by western blot analysis of PABPN1 in Jurkat, Hela, COS7, Raji lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
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WB
Figure 1 Anti-PRKRA Antibody (VS3-FY1813) in WB.
The PABPN1 expression in Jurkat, Hela, COS7, Raji lysates were detected using the anti-PABPN1 by western blot assay.
IHC
Figure 2 Anti-PRKRA Antibody (VS3-FY1813) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-PABPN1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human PACT (phospho-KR activating protein)/PRKRA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PRKRA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-8F7
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 34 kDa, while the observed molecular weight stands at 34 kDa.
- Related Disease
- Human breast cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- RAX; PACT; DYT16; HSD14
- Gene ID
- 8575
- UniProt ID
- O75569
- Sequence Similarities
- Belongs to the PRKRA family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells.
Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287.
Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.
(O75569-PRKRA_HUMAN)
Modification sites at PhosphoSitePlus (O75569)
Modification sites at neXtProt (NX_O75569)
Glycosylation from GlyGen (O75569) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011510365.1; XP_047302094.1; XP_054200276.1; XP_054200277.1; XP_054200278.1
- Function
- Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1528CT | Recombinant Mouse anti-Human PRKRA Monoclonal antibody (EML878) | IHC-P, WB |
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