Rabbit Anti-PRMT3 Recombinant Antibody (clone R01-7K2)
CAT#: VS3-FY1852
The R01-7K2 recombinant monoclonal antibody exhibits specific binding to human PRMT3. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P, IP. Applications Tested: The PRMT3 antibody has been tested by western blot analysis of PRMT3 in rat brain, C6, 3T3, Hela lysates and immunohistochemistry analysis of paraffin-embedded human lung cancer.
Tested Data
Gene Expression
WB
Figure 1 Anti-PRMT3 Antibody (VS3-FY1852) in WB.
The PRMT3 expression in rat Brain, C6, 3T3, Hela lysates were detected using the anti-PRMT3 by western blot assay.
IHC
Figure 2 Anti-PRMT3 Antibody (VS3-FY1852) in IHC.
Immunohistochemistry staining of paraffin-embedded human lung cancer using the anti-PRMT3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22083/56946_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22083/56946_A_7_3.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22083/56946_A_4_6.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185238-PRMT3
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Specifications
- Immunogen
- Synthetic peptide of human PRMT3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PRMT3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-7K2
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 60 kDa, while the observed molecular weight stands at 60 kDa.
- Related Disease
- Human lung cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- HRMT1L3
- Gene ID
- 10196
- UniProt ID
- O60678
- Sequence Similarities
- Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys187 (NX_O60678 [NX_O60678-1])
Modification sites at PhosphoSitePlus (O60678)
Glycosylation from GlyGen (O60678) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011518138.1; XP_047282183.1; XP_047282184.1; XP_054223404.1; XP_054223405.1
- Function
- May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRMT3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-480 | Recombinant Human Anti-Human PRMT3 Antibody, clone D483 | ELISA, IP-MS, ChIP | IgG |
| MOB-2342CT | Recombinant Mouse anti-Human PRMT3 Monoclonal antibody (C5) | WB, ICC, ELISA | |
| ZG-427C | Human Anti-PRMT3 Recombinant Antibody (ZG-427C) | ELISA, IP, ChIP-qPCR | Human IgG1, κ |
| VS11-QX123 | Human Anti-PRMT3 Recombinant Antibody (clone RAB-C372) | ELISA, IP-MS, ChIP | Human IgG1, κ |
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