Mouse Anti-PrP Recombinant Antibody (clone sc4.1)
CAT#: VS-0623-WK5
The antibody clone sc4.1 was produced by immunizing mouse with recombinant PrP. sc4.1 binds a PrP epitope overlapping that of monoclonal antibody 6H4. It can be used for detection of mouse PrP in ELISA, WB and DB assays.
Published Data
WB
Figure 1 Western blot analysis of anti-PrP scFv protein (clone 4.1A, lane 2).
Supernatants derived from cells transfected with PSA (lane 3) or GFP (lane 1) served as positive and negative controls.
DB
Figure 2 Dot blot analysis of anti-PrP scFv protein (clone 4.1A).
Recombinant PrP (PrPREC; 200 ng) was spotted onto nitrocellulose and incubated with medium derived from RD-4 cells stably expressing anti-PrP scFv (clone 4.5 and clone 4.1), GFP, and PSA.
ELISA
Figure 3 Affinity-purified sc4.1 resulted in concentration-dependent binding to PrPᒼ (an abnormal form of the cellular prion protein) as assessed by ELISA.
Inhib
Figure 4 Inhibition analysis of scFv (clone 4.1) binding to Recombinant PrP by ELISA.
Pretreatment of Recombinant PrP-coated ELISA plates with increasing amounts of monoclonal antibody 6H4 (recognizing mouse PrP144-152) inhibited binding of 4 μg purified scFvs (clone 4.1) to PrPREC (squares), while anti-PrP antibody POM2 (which recognizes an amino-terminal epitope of PrPC) did not alter PrP reactivity of sc 4.1 (triangles).
Inhib
Figure 5 Inhibition analysis of scFv (clone 4.1) binding to Recombinant PrP by WB.
Monoclonal antibody 6H4, but not the IgG isotype control, antagonized binding to Recombinant PrP when administered prior to incubation with sc4.1.
ICW
Figure 6 Anti-PrP scFvs rescue chronically scrapie-infected ScN2a cells by In-cell Western assay.
Decrease in PrPSc content in ScN2a cells cocultured with stably transfected RD-4 cells producing large amounts of anti-PrP scFvs (4.1 high, in duplicate, lanes 1 and 2, upper row). Ethidium bromide staining demonstrates the presence of equal amounts of cells per well upon transfer onto nitrocellulose membrane (lower row).
FuncS
Figure 7 PrPSc load after 12 days of treatment with anti-PrP-scFv.
After 3 weeks of coculturing ScN2a cells with anti-PrP-scFv-producing RD-4 cells (clone 4.1), ScN2a cells were left in culture for three further passages (corresponding to 12 days) without further anti-PrP scFvs treatment. A strong reduction of PrPSc was observable in ScN2a cells that had previously been exposed to RD-4 cells producing high levels of anti-PrP scFv (clone 4.1 high, in duplicate; lanes 3 and 4).
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Specifications
- Immunogen
- Recombinant PrP
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Mouse PrP
- Species Reactivity
- Mouse
- Clone
- sc4.1
- Applications
- ELISA, WB, DB, Inhib, ICW, FuncS
- Conjugate
- Unconjugated
- Related Disease
- Prion diseases
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody specificly binds to PrP by western blot, dot blot, inhibition, enzyme-linked immunoassay, and in-cell western assay.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-815 | Recombinant Anti-PRP Antibody | ELISA, WB, FuncS | IgG |
| MHH-815 | Recombinant Human Anti-PRP Antibody | IF, FuncS | IgG |
| HPAB-M0613-YC | Human Anti-PrPᶜ Recombinant Antibody (HPAB-M0613-YC) | FC, HTRF assay | Human IgG |
| FAMAB-0039WJ | Human Anti-PRP Recombinant Antibody (clone SB5D6) | ELISA, WB | Human IgA1, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-815-F(E) | Recombinant Anti-PRP Antibody Fab Fragment | WB, IP, FuncS | Fab |
| MHH-815-F(E) | Recombinant Human Anti-PRP Antibody Fab Fragment | WB, FC, FuncS | Fab |
| HPAB-M0613-YC-F(E) | Human Anti-PrPᶜ Recombinant Antibody; Fab Fragment (HPAB-M0613-YC-F(E)) | FC, HTRF assay | Human Fab |
| FAMAB-0039WJ-F(E) | Human Anti-PRP Recombinant Antibody (clone SB5D6); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0040WJ-F(E) | Human Anti-PRP Recombinant Antibody (clone ED8.4); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-815-S(P) | Recombinant Anti-PRP Antibody scFv Fragment | WB, FC, FuncS | scFv |
| MHH-815-S(P) | Recombinant Human Anti-PRP Antibody scFv Fragment | Neut, WB, FuncS | scFv |
| HPAB-M0613-YC-S(P) | Human Anti-PrPᶜ Recombinant Antibody; scFv Fragment (HPAB-M0613-YC-S(P)) | FC, HTRF assay | Human scFv |
| FAMAB-0039WJ-S(P) | Human Anti-PRP Recombinant Antibody (clone SB5D6); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0040WJ-S(P) | Human Anti-PRP Recombinant Antibody (clone ED8.4); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0040WJ | Human Anti-PRP Recombinant Antibody (clone ED8.4) | ELISA, WB | Human IgM, κ |
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