Rabbit Anti-PRPF4 Recombinant Antibody (clone R07-3C1) (CAT#: VS3-FY2071)
The R07-3C1 recombinant monoclonal antibody exhibits specific binding to human PRPF4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The antibody has the positive detection of PRPF4 by western blot analysis of PRPF4 in Raw264.7, C6 lysates and western blot analysis of PRPF4 in Hela, A549, HL-60 lysates.
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WB
Figure 1 Anti-PRPF4 Antibody (VS3-FY2071) in WB.
The PRPF4 expression in Raw264.7, C6 lysates were detected using the anti-PRPF4 by western blot assay.
WB
Figure 2 Anti-PRPF4 Antibody (VS3-FY2071) in WB.
The PRPF4 expression in Hela, A549, HL-60 lysates were detected using the anti-PRPF4 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human PRPF4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PRPF4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-3C1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 58 kDa, while the observed molecular weight stands at 58 kDa.
- Related Disease
- Retinitis Pigmentosa 70 and Retinitis Pigmentosa
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- PRP4; RP70; HPRP4; Prp4p; HPRP4P; SNRNP60
- Gene ID
- 9128
- UniProt ID
- O43172
- Cellular Localization
- Nucleus, Spliceosome
- Post Translation Modifications
- Ubiquitination at Lys159 (NX_O43172 [NX_O43172-1])
Modification sites at PhosphoSitePlus (O43172)
Glycosylation from GlyGen (O43172) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001231855.1; NP_001309195.1; NP_001309196.1; NP_004688.2
- Function
- Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1144 | AbPlus™ Anti-PRPF4 Magnetic Beads (VS-0724-YC1144) | IP, Protein Purification |
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