Rabbit Anti-PRPF4 Recombinant Antibody (clone R07-3C1) (CAT#: VS3-FY2071)
The R07-3C1 recombinant monoclonal antibody exhibits specific binding to human PRPF4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The antibody has the positive detection of PRPF4 by western blot analysis of PRPF4 in Raw264.7, C6 lysates and western blot analysis of PRPF4 in Hela, A549, HL-60 lysates.
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WB
Figure 1 Anti-PRPF4 Antibody (VS3-FY2071) in WB.
The PRPF4 expression in Raw264.7, C6 lysates were detected using the anti-PRPF4 by western blot assay.
WB
Figure 2 Anti-PRPF4 Antibody (VS3-FY2071) in WB.
The PRPF4 expression in Hela, A549, HL-60 lysates were detected using the anti-PRPF4 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human PRPF4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PRPF4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-3C1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 58 kDa, while the observed molecular weight stands at 58 kDa.
- Related Disease
- Retinitis Pigmentosa 70 and Retinitis Pigmentosa
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- PRP4; RP70; HPRP4; Prp4p; HPRP4P; SNRNP60
- Gene ID
- 9128
- UniProt ID
- O43172
- Cellular Localization
- Nucleus, Spliceosome
- Post Translation Modifications
- Ubiquitination at Lys159 (NX_O43172 [NX_O43172-1])
Modification sites at PhosphoSitePlus (O43172)
Glycosylation from GlyGen (O43172) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001231855.1; NP_001309195.1; NP_001309196.1; NP_004688.2
- Function
- Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1144 | AbPlus™ Anti-PRPF4 Magnetic Beads (VS-0724-YC1144) | IP, Protein Purification |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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