Rabbit Anti-Ptf1A Recombinant Antibody (clone R04-7B1)
CAT#: VS3-FY1615
Clone R04-7B1 is a rabbit recombinant monoclonal antibody that with high affinity for human Ptf1A. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IP. This PTF1A antibody has been teted positive by western blot analysis of PTF1A in K562, C6 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-Ptf1A Antibody (VS3-FY1615) in WB.
The PTF1A expression in K562, C6 lysates were detected using the anti-PTF1A by western blot assay.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22612/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enriched (U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168267-PTF1A/subcellular
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Specifications
- Immunogen
- Recombinant mouse PTF1A protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat Ptf1A
- Species Reactivity
- Human, Rat
- Clone
- R04-7B1
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 35 kDa, while the observed molecular weight stands at 48 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- PTF1A; PACA; PAGEN2; PTF1-p48; bHLHa29; pancreas specific transcription factor; 1a; p48; pancreas associated transcription factor 1a
- Gene ID
- 19213
- UniProt ID
- Q9QX98
- Cellular Localization
- Cytoplasm, Nucleus
- Protein Refseq
- NP_061279.2
- Function
- Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PTF1A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1718CT | Recombinant Mouse anti-Human PTF1A Monoclonal antibody (EML1494) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5825 | Anti-PTF1A Immunohistochemistry Kit | IHC |
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