Rabbit Anti-PXN (pY118) Recombinant Antibody (clone R04-1V5)
CAT#: VS3-WK2150
This product is a rabbit recombinant antibody that is specific to phosphorylated PXN. The target of this antibody is phospho-PXN (Tyr118). The clone R04-1V5 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of PXN (pTyr118) observed in WB experiments using this antibody is 68 kDa.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51309/795_G6_5_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_9_3.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_B_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089159-PXN/subcellular
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr118 of human, mouse and rat PXN
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-1V5
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "PXN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q) | WB, ELISA, IF, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q); scFv Fragment | WB, ELISA, IF, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-674 | Human Anti-PXN Recombinant Antibody (clone CBL19Q) | WB, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-675 | Human Anti-PXN Recombinant Antibody (clone CBL35Q) | WB, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-668 | Human Anti-PXN Recombinant Antibody (clone CBL19Q); Fab Fragment | WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-669 | Human Anti-PXN Recombinant Antibody (clone CBL35Q); Fab Fragment | WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-668 | Human Anti-PXN Recombinant Antibody (clone CBL19Q); scFv Fragment | WB, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-669 | Human Anti-PXN Recombinant Antibody (clone CBL35Q); scFv Fragment | WB, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1558CT | Recombinant Mouse anti-Human PXN Monoclonal antibody (N203) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2947 | Hi-Affi™ Recombinant Rabbit Anti-PXN Monoclonal Antibody (DS2947AB) | FC, ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1178-CN | Recombinant Rabbit Anti-PXN Monoclonal Antibody (CBACN-434) | WB, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5893 | Anti-PXN Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5894 | Anti-Mouse PXN Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5895 | Anti-Rat PXN Immunohistochemistry Kit | IHC |
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