Rabbit Anti-PXN Recombinant Antibody (clone R07-1B4)
CAT#: VS3-FY2059
This rabbit recombinant monoclonal antibody R07-5E0 shows high affinity for human PXN. It cross-reacts with rat, hamster. It is suitable for use in a variety of assays, including WB, IHC-P, IP. Western blot demonstrated that R07-1B4 recognized a target protein with a molecular weight of 65 kDa.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51309/795_G6_5_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_9_3.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_B_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089159-PXN/subcellular
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WB
Figure 1 Anti-PXN Antibody (VS3-FY2059) in WB.
The Paxillin expression in Hela lysates were detected using the anti-Paxillin by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human paxillin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat, Hamster PXN
- Species Reactivity
- Human, Rat, Hamster
- Clone
- R07-1B4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 65 kDa, while the observed molecular weight stands at 65 kDa.
- Related Disease
- Schwannomatosis, Vestibular and Prostate Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Paxillin
- Gene ID
- 5829
- UniProt ID
- P49023
- Sequence Similarities
- Belongs to the paxillin family.
- Cellular Localization
- Cell junction, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Phosphorylated by MAPK1/ERK2 ()., Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389).
- Protein Refseq
- NP_001074324.1; NP_001230685.1; NP_002850.2
- Function
- Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PXN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q); scFv Fragment | WB, ELISA, IF, FuncS | Human scFv |
| PSBL-668 | Human Anti-PXN Recombinant Antibody (clone CBL19Q); scFv Fragment | WB, FuncS | Human scFv |
| PSBL-669 | Human Anti-PXN Recombinant Antibody (clone CBL35Q); scFv Fragment | WB, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
| PFBL-668 | Human Anti-PXN Recombinant Antibody (clone CBL19Q); Fab Fragment | WB, FuncS | Human Fab |
| PFBL-669 | Human Anti-PXN Recombinant Antibody (clone CBL35Q); Fab Fragment | WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2947 | Hi-Affi™ Recombinant Rabbit Anti-PXN Monoclonal Antibody (DS2947AB) | FC, ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1491CQ | Mouse Anti-PXN Recombinant Antibody (clone LD4 sAB) | WB, ELISA | Mouse IgG1 |
| FAMAB-1492CQ | Mouse Anti-PXN Recombinant Antibody (clone LD2 sAB) | WB, ELISA | Mouse IgG1 |
| ZG-0537U | Rabbit Anti-Phospho-PXN (Y118) Recombinant Antibody (clone 1F11) | ELISA, WB | Rabbit IgG |
| ZG-0538U | Rabbit Anti-PXN Recombinant Antibody (clone 2G8) | ELISA | Rabbit IgG |
| VS3-CJ984 | Rabbit Anti-PXN Recombinant Antibody (VS3-CJ984) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5893 | Anti-PXN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5894 | Anti-Mouse PXN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5895 | Anti-Rat PXN Immunohistochemistry Kit | IHC |
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