Rabbit Anti-PXN Recombinant Antibody (clone R07-1B4) (CAT#: VS3-FY2059)
This rabbit recombinant monoclonal antibody R07-5E0 shows high affinity for human PXN. It cross-reacts with rat, hamster. It is suitable for use in a variety of assays, including WB, IHC-P, IP. Western blot demonstrated that R07-1B4 recognized a target protein with a molecular weight of 65 kDa.
Specific Inquiry
WB
Figure 1 Anti-PXN Antibody (VS3-FY2059) in WB.
The Paxillin expression in Hela lysates were detected using the anti-Paxillin by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human paxillin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat, Hamster PXN
- Species Reactivity
- Human, Rat, Hamster
- Clone
- R07-1B4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 65 kDa, while the observed molecular weight stands at 65 kDa.
- Related Disease
- Schwannomatosis, Vestibular and Prostate Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Paxillin
- Gene ID
- 5829
- UniProt ID
- P49023
- Sequence Similarities
- Belongs to the paxillin family.
- Cellular Localization
- Cell junction, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Phosphorylated by MAPK1/ERK2 ()., Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389).
- Protein Refseq
- NP_001074324.1; NP_001230685.1; NP_002850.2
- Function
- Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PXN"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q) | WB, ELISA, IF, FuncS | Human IgG |
| FAMAB-1491CQ | Mouse Anti-PXN Recombinant Antibody (clone LD4 sAB) | WB, ELISA | Mouse IgG1 |
| FAMAB-1492CQ | Mouse Anti-PXN Recombinant Antibody (clone LD2 sAB) | WB, ELISA | Mouse IgG1 |
| VS3-CJ984 | Rabbit Anti-PXN Recombinant Antibody (VS3-CJ984) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-WK2150 | Rabbit Anti-PXN (pY118) Recombinant Antibody (clone R04-1V5) | WB | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-FY2059. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
-1.jpg)
Application: FC, FRET, Internalization
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: FC, IHC, Cyt, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
