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  • Rabbit Anti-RAB22A Recombinant Antibody (clone R07-3A8)

Rabbit Anti-RAB22A Recombinant Antibody (clone R07-3A8) (CAT#: VS3-FY1864)

Clone R07-3A8 is a rabbit IgG recombinant monoclonal antibody that exhibits specific detecting to human RAB22A protein, a calculated weight of 22 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R07-3A8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).


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Normal Tissue
Figure 1 IHC staining of human rectum
Figure 1 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66920/ihc_selected.jpg
Figure 2 Cerebral cortex
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66920/146781_B_7_5.jpg
Figure 3 Colon
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66920/146781_A_9_3.jpg
Figure 4 Liver
Figure 4 Liver
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66920/146781_A_7_4.jpg
Figure 5 Pancreas
Figure 5 Pancreas
(Exocrine glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66920/146781_A_2_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66920/146781_A_7_5.jpg
Figure 7 Testis
Figure 7 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66920/146781_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124209-RAB22A

Specifications

  • Immunogen
  • Synthetic peptide of human RAB22A
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat RAB22A
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R07-3A8
  • Applications
  • Western Blot, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 22 kDa.
  • Related Disease
  • Exocervical Carcinoma

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • RAB22A, Member RAS Oncogene Family; Rab-22; Ras-Related Protein Rab-22A; GTP-Binding Protein RAB22A; RAB22
  • Sequence Similarities
  • Belongs to the small GTPase superfamily. Rab family.
  • Cellular Localization
  • Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Membrane
  • Post Translation Modifications
  • Modification sites at PhosphoSitePlus (Q9UL26)
    Modification sites at neXtProt (NX_Q9UL26)
  • Function
  • Plays a role in endocytosis and intracellular protein transport. Mediates trafficking of TF from early endosomes to recycling endosomes.
    Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth.
    Binds GTP and GDP and has low GTPase activity. Alternates between a GTP-bound active form and a GDP-bound inactive form.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "RAB22A"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2958 Hi-Affi™ Recombinant Rabbit Anti-RAB22A Monoclonal Antibody (DS2958AB) WB, ICC, IP IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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