Rabbit Anti-RAB29 Recombinant Antibody (clone R08-3C0)
CAT#: VS3-FY2074
The Rab29 antibody detected positive in western blot analysis of RAB29 in Hela, A549, HL-60, U251 lysates. The recombinant monoclonal antibody shows specific binding to human RAB29. It is suitable for use in a variety of assays, including WB, IP.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HDLM-2
Immunofluorescent staining of human cell line HDLM-2 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26303/1959_C2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26303/55137_B_4_5_selected.jpg
Normal Tissue
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows moderate positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20822/46847_A_8_3_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26303/55137_B_8_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26303/55137_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26303/55137_A_5_6.jpg
Normal Tissue
Figure 7 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26303/55137_B_8_1.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117280-RAB29
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WB
Figure 1 Anti-RAB29 Antibody (VS3-FY2074) in WB.
The RAB29 expression in Hela, A549, HL-60, U251 lysates were detected using the anti-Rab29 by western blot assay.
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Specifications
- Immunogen
- Recombinant human RAB29 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RAB29
- Species Reactivity
- Human
- Clone
- R08-3C0
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 23 kDa, while the observed molecular weight stands at 23 kDa.
- Related Disease
- Parkinson Disease 21 and Kufor-Rakeb Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- RAB29, Member RAS Oncogene Family; RAB7, Member RAS Oncogene Family-Like 1; Ras-Related Protein Rab-29; Rab-7-Like Protein 1; RAB7L1; Ras-Related Protein Rab-7L1; RAB7L
- Gene ID
- 8934
- UniProt ID
- O14966
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rab family.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Vacuole
- Post Translation Modifications
- In case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles.
In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells (PubMed:22042847).
(O14966-RAB7L_HUMAN)
Modification sites at PhosphoSitePlus (O14966)
Modification sites at neXtProt (NX_O14966)
- Protein Refseq
- XP_005245626.1; XP_005245627.1; XP_005245628.1; XP_006711668.1; XP_006711669.1
- Function
- The small GTPases Rab are key regulators in vesicle trafficking.
Essential for maintaining the integrity of the endosome-trans-Golgi network structure (By similarity).
Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RAB29"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1194CT | Recombinant Mouse anti-Human RAB7L1 Monoclonal antibody (EML877) | WB | |
| VS3-CJ133 | Mouse Anti-RAB29 Recombinant Antibody (VS3-CJ133) | WB | Mouse IgG1 |
| VS3-FY2855 | Mouse Anti-RAB29 Recombinant Antibody (VS3-FY2855) | WB | Mouse IgG |
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