Rabbit Anti-RASGRF1 Recombinant Antibody (clone R06-5H5) (CAT#: VS3-FY1680)
This product is a extraordinary rabbit recombinant monoclonal antibody that shows specific binding to human and rat RASGRF1. The calculated molecular weight of the antigen is 145 kDa; the observed molecular weight of the protein is 145 kDa. The R06-5H5 is recommended for dilution with western blot (dilution 1/500-1/1000).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41965/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41965/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10413/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10413/if_selected.jpg
Figure 3 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41965/90759_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41965/90759_A_9_8.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41965/90759_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41965/90759_A_9_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41965/90759_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41965/90759_A_4_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (A-431, BEWO, Karpas-707, RPMI-8226, U-2 OS)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000058335-RASGRF1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000058335-RASGRF1
Specifications
- Immunogen
- Synthetic peptide of human CDC25
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat RASGRF1
- Species Reactivity
- Human, Rat
- Clone
- R06-5H5
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 145 kDa, while the observed molecular weight stands at 145 kDa.
- Related Disease
- Bleeding Disorder, Platelet-Type, 18 and Degenerative Myopia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Ras Protein Specific Guanine Nucleotide Releasing Factor 1; Ras-Specific Nucleotide Exchange Factor CDC25; Guanine Nucleotide-Releasing Protein; Ras-GRF1; CDC25; GRF1; GNRP; Ras-Specific Guanine Nucleotide-Releasing Factor, CDC25 Homolog; Ras-Specific Guanine Nucleotide-Releasing Factor 1
- Gene ID
- 5923
- UniProt ID
- Q13972
- Cellular Localization
- cytosol, growth cone, neuron projection, plasma membrane
- Post Translation Modifications
- Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.
Ubiquitinated and degraded following phosphorylation by PLK2.
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.
- Protein Refseq
- XP_011520168.1; XP_016877944.1; XP_016877945.1; XP_016877946.1; XP_016877947.1
- Function
- Promotes the exchange of Ras-bound GDP by GTP.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3009 | Hi-Affi™ Recombinant Rabbit Anti-RASGRF1 Monoclonal Antibody (DS3009AB) | WB | IgG |
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