Rabbit Anti-RBBP5 Recombinant Antibody (VS13-YC988) (CAT#: VS13-YC988)
The Rabbit Anti-RBBP5 Recombinant Antibody is specific for human, mouse and rat RBBP5. RBBP5, similar to RBBP4, regulates transcription through association with retinoblastoma proteins and its gene expression. The anti-RBBP5 antibody can be used for Western blot (1/1000).
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49042/if_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49042/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_5_6_selected.jpg
(Immunohistochemical staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_5_6_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49042/748_C10_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49042/748_C10_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49042/728_C10_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49042/728_C10_1_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_B_9_5.jpg
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_B_9_5.jpg
Figure 6 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_8_3.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_8_3.jpg
Figure 7 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_9_4.jpg
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_9_4.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_8_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_8_5.jpg
Figure 9 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_5_6.jpg
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58085/131532_A_5_6.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117222-RBBP5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117222-RBBP5
Specifications
- Immunogen
- Recombinant human RbBP5, aa 431-480.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat RBBP5
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000
Target
- Alternative Names
- RB Binding Protein 5, Histone Lysine Methyltransferase Complex Subunit; Retinoblastoma-Binding Protein RBQ-3; Retinoblastoma-Binding Protein 5; RBBP-5; RBQ3; SWD1, Set1c WD40 Repeat Protein, Homolog (S. Cerevisiae); SWD1, Set1c WD40 Repeat Protein, Homolog; Retinoblastoma Binding Protein 5; SWD1;
- Gene ID
- 5929
- UniProt ID
- Q15291
- Long Name
- RB Binding Protein 5, Histone Lysine Methyltransferase Complex Subunit
- Cellular Localization
- Nucleus
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-493 | Recombinant Human Anti-Human RBBP5 Antibody, clone D487 | ELISA, IP-MS | IgG |
| ZG-061R | Mouse Anti-RBBP5 Recombinant Antibody (ZG-061R) | WB, ICC | Mouse IgG |
| VS3-QX961 | Mouse Anti-RBBP5 Recombinant Antibody (clone 1C9-1C9-H10) | WB, IF | Mouse IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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