Mouse Anti-RBCK1 Recombinant Antibody (VS3-FY2862) (CAT#: VS3-FY2862)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human, Mouse and Rat RBCK1

• Species Reactivity
• Human, Mouse, Rat

• Applications
• Western Blot

• Conjugate
• Unconjugated, FITC

• MW
• The molecular weight of the target protein is approximately 57.6 kDa.

• Related Disease
• Polyglucosan Body Myopathy 1 With Or Without Immunodeficiency and Glycogen Storage Disease Iv

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/2000

• Alternative Names
• RANBP2-Type And C3HC4-Type Zinc Finger Containing 1; Heme-Oxidized IRP2 Ubiquitin Ligase 1; RING-Type E3 Ubiquitin Transferase HOIL-1; Hepatitis B Virus X-Associated Protein 4; HBV-Associated Factor 4; RING Finger Protein 54; C20orf18; UBCE7IP3; HOIL-1; RNF54; XAP3; XAP4; RanBP-Type And C3HC4-Type Zinc Finger-Containing Protein 1

• Gene ID
• 10616

• UniProt ID
• Q9BYM8

• Sequence Similarities
• Belongs to the RBR family.

• Cellular Localization
• cytosol, nucleus

• Post Translation Modifications
• Auto-ubiquitinated.
  Auto-ubiquitination leads to degradation by the proteasome (By similarity).
  (Q9BYM8-HOIL1_HUMAN)
  Phosphorylated.
  In vitro, phosphorylation inhibits auto-ubiquitination activity (By similarity).
  (Q9BYM8-HOIL1_HUMAN)
  (Microbial infection) Ubiquitinated by S.flexneri E3 ubiquitin-protein ligases IpaH1.4 and IpaH2.5, leading to its degradation by the proteasome, thereby preventing formation of the bacterial ubiquitin coat and activation of innate immunity (PubMed:27572974, 35294289). (Q9BYM8-HOIL1_HUMAN)
  Ubiquitination at Lys29, Lys158, Lys174, Lys254, and Lys342 (NX_Q9BYM8 [NX_Q9BYM8-1])
  Modification sites at PhosphoSitePlus

• Protein Refseq
• XP_011527440.1; XP_011527441.1; XP_011527442.1; XP_047295789.1

• Function
• Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination (PubMed:12629548).
  Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome (PubMed:17449468, 18711448).
  Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed:17006537, 21455173, 21455180, 21455181, 19136968).
  LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways.

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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