Rabbit Anti-RGS6 Recombinant Antibody (clone R02-1D3)
CAT#: VS3-FY2079
The anti-RGS6 antibody detected positive in western blot analysis of RGS6 in A549, HL-60, U2OS lysates. The recombinant monoclonal antibody is designed to compete for binding to human RGS6. The R02-1D3 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Tested Data
Gene Expression
WB
Figure 1 Anti-RGS6 Antibody (VS3-FY2079) in WB.
The RGS6 expression in A549, HL-60, U2OS lysates were detected using the anti-RGS6 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3067/1848_H2_18_cr5abb8cae97a2e_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3067/8530_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3067/8530_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3067/8530_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3067/8530_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3067/8530_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3067/8530_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (HEL, Karpas-707)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182732-RGS6
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Specifications
- Immunogen
- Synthetic peptide of human RGS6
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RGS6
- Species Reactivity
- Human
- Clone
- R02-1D3
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 54 kDa, while the observed molecular weight stands at 54 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Regulator Of G Protein Signaling 6; Regulator Of G-Protein Signalling 6; Regulator Of G-Protein Signaling 6; S914; HA117; GAP
- Gene ID
- 9628
- UniProt ID
- P49758
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus
- Protein Refseq
- NP_001191345.1; NP_001191346.1; NP_001191347.1
- Function
- Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS6/GNB5 dimer enhances GNAO1 GTPase activity.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RGS6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0506MZ | Chicken Anti-RGS6 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3063 | Hi-Affi™ Recombinant Rabbit Anti-RGS6 Monoclonal Antibody (DS3063AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS4-CJ102 | Recombinant Rabbit Anti-RGS6 Antibody (clone 12H5) | ELISA, IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1913 | Anti-RGS6 Immunohistochemistry Kit | IHC |
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