Human Anti-Rh(D) Recombinant Antibody (clone F5) (CAT#: FAMAB-0089WJ)
This product is a human monoclonal antibody IgG1, λ targeting Rhesus (Rh) antigen.
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FC
Figure 1 Flow cytometric analysis of HEK293 cells expressing recombinantRhAG and RhD antigen.
The human MAbanti-D F5was used to compare RhDantigen expression on hygromycin- and G418-resistant HEK293/RhAG/RhD cells(____) and HEK293/RhD cells (. .). In dot-plot representations, the R2 region wasdefined as a positive region in which no signal was detected with negative controls(mouse IgG1 andhuman anti-K1MAb).
Mouro-Chanteloup, I., D'Ambrosio, A. M., Gane, P., Le Van Kim, C., Raynal, V., Dhermy, D., ... & Colin, Y. (2002). Cell-surface expression of RhD blood group polypeptide is posttranscriptionally regulated by the RhAG glycoprotein. Blood, The Journal of the American Society of Hematology, 100(3), 1038-1047.
FC
Figure 2 Flow cytometric analysis of the enrichment of K562 cells expressingrecombinant RhD antigen by magnetic-bead sorting.
The human MAb anti-D F5was used to compare RhD antigen expression on G418-resistant K562/RhD cellsbefore and after magnetic bead sorting, as described in "Materials and methods."K562/Lu stablyexpressing therecombinant Lu blood groupglycoprotein wasused asnegative control.
Mouro-Chanteloup, I., D'Ambrosio, A. M., Gane, P., Le Van Kim, C., Raynal, V., Dhermy, D., ... & Colin, Y. (2002). Cell-surface expression of RhD blood group polypeptide is posttranscriptionally regulated by the RhAG glycoprotein. Blood, The Journal of the American Society of Hematology, 100(3), 1038-1047.
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG1, λ
- Specificity
- Rhesus (Rh) antigen
- Species Reactivity
- Rhesus
- Clone
- F5
- Applications
- ELISA, WB, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Rh(D) antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry.
Target
- Alternative Names
- Rhesus D; Rh(D); RH; Rh4; RH30; RhII; RhPI; DIIIc; RHCED; RHDel; RHPII; RhDCw; CD240D; RHXIII; RHDVA(TT); RhK562-II
- Gene ID
- 6007
- UniProt ID
- Q02161
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0089WJ-F(E) | Human Anti-Rh(D) Recombinant Antibody (clone F5); Fab Fragment | ELISA, WB | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0089WJ-S(P) | Human Anti-Rh(D) Recombinant Antibody (clone F5); scFv Fragment | ELISA, WB | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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