Rabbit Anti-RIPK2 Recombinant Antibody (clone R87-7G-8) (CAT#: VS3-FY2190)

Clone R87-7G-8 is a IgG recombinant monoclonal antibody that exhibits a high affinity for human RIPK2 protein, a calculated weight of 83 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R87-7G-8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Normal Tissue
RNA Expression

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat RIPK2
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R87-7G-8
  • Applications
  • Western Blot
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 61kDa, while the observed molecular weight stands at 61kDa.
  • Related Disease
  • Crohn's Disease and Blau Syndrome

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000

Target

  • Alternative Names
  • Receptor Interacting Serine/Threonine Kinase 2; CARD-Containing IL-1 Beta ICE-Kinase; Receptor-Interacting Protein 2; Tyrosine-Protein Kinase RIPK2; EC 2.7.11.1; CARDIAK; RIP-2; RIP2; RICK; Receptor-Interacting Protein (RIP)-Like Interacting Caspase-Like Apoptosis Regulatory Protein (CLARP) Kinase; CARD-Containing Interleukin-1 Beta-Converting Enzyme (ICE)-Associated Kinase; CARD-Containing Interleukin-1 Beta-Converting Enzyme-Associated Kinase
  • Sequence Similarities
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
  • Cellular Localization
  • Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane
  • Post Translation Modifications
  • Polyubiquitinated via both 'Lys-63'- and 'Met-1'-linked polyubiquitin following recruitment by NOD1 or NOD2, creating docking sites for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling (PubMed:22607974, 29452636, 30026309).
    'Lys-63'-linked polyubiquitination by XIAP is essential for NOD2 signaling and promotes recruitment of the LUBAC complex (PubMed:22607974, 29452636, 30026309).
    Also polyubiquitinated with 'Lys-63'-linked chains by PELI3, BIRC2/c-IAP1 and BIRC3/c-IAP2 (PubMed:19464198, 21931591).
    Ubiquitinated on Lys-209 via 'Lys-63'-linked by ITCH (PubMed:18079694, 19592251).
    Undergoes 'Lys-63'-linked deubiquitination by MYSM1 to attenuate NOD2-mediated inflammation and tissue damage ().
    Polyubiquitinated with 'Lys-63'-linked chains in response to Shigella infection, promoting its SQSTM1/p62-dependent autophagic degradation (PubMed:36221902).
    Undergoes 'Met-1'-linked polyubiquitination; the head-to-tail linear polyubiquitination is mediated by the LUBAC complex in response to NOD2 stimulation 'Met-1'-linked polyubiquitination (PubMed:23806334).
    'Lys-63'-linked polyubiquitination by XIAP is required for recruimtent of the LUBAC complex and subsequent (PubMed:22607974).
    Linear polyubiquitination is restricted by FAM105B/otulin, probably to limit NOD2-dependent pro-inflammatory signaling activation of NF-kappa-B (PubMed:23806334).
    Ubiquitination at Lys-503 by ZNRF4 via 'Lys-48'-linked polyubiquitination promotes RIPK2 degradation by the proteasome; ubiquitination by ZNRF4 takes place during both acute and NOD2 tolerance conditions (PubMed:28656966).
  • Function
  • Acts as a key effector of NOD1 and NOD2 signaling pathways: upon activation by bacterial peptidoglycans, NOD1 and NOD2 oligomerize and recruit RIPK2 via CARD-CARD domains, leading to the formation of RIPK2 filaments.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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