Rabbit Anti-RIPK2 Recombinant Antibody (clone R87-7G-8) (CAT#: VS3-FY2190)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat RIPK2

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R87-7G-8

• Applications
• Western Blot

• Conjugate
• Unconjugated

• MW
• The calculated weight of the target protein is approximately 61kDa, while the observed molecular weight stands at 61kDa.

• Related Disease
• Crohn's Disease and Blau Syndrome

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS (pH 7.4), 150 mM NaCl, 50% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Receptor Interacting Serine/Threonine Kinase 2; CARD-Containing IL-1 Beta ICE-Kinase; Receptor-Interacting Protein 2; Tyrosine-Protein Kinase RIPK2; EC 2.7.11.1; CARDIAK; RIP-2; RIP2; RICK; Receptor-Interacting Protein (RIP)-Like Interacting Caspase-Like Apoptosis Regulatory Protein (CLARP) Kinase; CARD-Containing Interleukin-1 Beta-Converting Enzyme (ICE)-Associated Kinase; CARD-Containing Interleukin-1 Beta-Converting Enzyme-Associated Kinase

• Gene ID
• 8767

• UniProt ID
• O43353

• Sequence Similarities
• Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

• Cellular Localization
• Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane

• Post Translation Modifications
• Polyubiquitinated via both 'Lys-63'- and 'Met-1'-linked polyubiquitin following recruitment by NOD1 or NOD2, creating docking sites for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling (PubMed:22607974, 29452636, 30026309).
  'Lys-63'-linked polyubiquitination by XIAP is essential for NOD2 signaling and promotes recruitment of the LUBAC complex (PubMed:22607974, 29452636, 30026309).
  Also polyubiquitinated with 'Lys-63'-linked chains by PELI3, BIRC2/c-IAP1 and BIRC3/c-IAP2 (PubMed:19464198, 21931591).
  Ubiquitinated on Lys-209 via 'Lys-63'-linked by ITCH (PubMed:18079694, 19592251).
  Undergoes 'Lys-63'-linked deubiquitination by MYSM1 to attenuate NOD2-mediated inflammation and tissue damage ().
  Polyubiquitinated with 'Lys-63'-linked chains in response to Shigella infection, promoting its SQSTM1/p62-dependent autophagic degradation (PubMed:36221902).
  Undergoes 'Met-1'-linked polyubiquitination; the head-to-tail linear polyubiquitination is mediated by the LUBAC complex in response to NOD2 stimulation 'Met-1'-linked polyubiquitination (PubMed:23806334).
  'Lys-63'-linked polyubiquitination by XIAP is required for recruimtent of the LUBAC complex and subsequent (PubMed:22607974).
  Linear polyubiquitination is restricted by FAM105B/otulin, probably to limit NOD2-dependent pro-inflammatory signaling activation of NF-kappa-B (PubMed:23806334).
  Ubiquitination at Lys-503 by ZNRF4 via 'Lys-48'-linked polyubiquitination promotes RIPK2 degradation by the proteasome; ubiquitination by ZNRF4 takes place during both acute and NOD2 tolerance conditions (PubMed:28656966).

• Protein Refseq
• XP_011515659.1; XP_054217414.1; NP_001362289.1; NP_003812.1

• Function
• Acts as a key effector of NOD1 and NOD2 signaling pathways: upon activation by bacterial peptidoglycans, NOD1 and NOD2 oligomerize and recruit RIPK2 via CARD-CARD domains, leading to the formation of RIPK2 filaments.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody