Rabbit Anti-RIPK2 Recombinant Antibody (clone R87-7G-8)
CAT#: VS3-FY2190
Clone R87-7G-8 is a IgG recombinant monoclonal antibody that exhibits a high affinity for human RIPK2 protein, a calculated weight of 83 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R87-7G-8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15273/125_F4_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in Umbrella cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15273/ihc_selected.jpg
Normal Tissue
Figure 3 Placenta
Decidual cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15273/38750_A_2_7.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15273/38750_A_7_8.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15273/38750_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15273/38750_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (hTERT-HME1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104312-RIPK2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat RIPK2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R87-7G-8
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 61kDa, while the observed molecular weight stands at 61kDa.
- Related Disease
- Crohn's Disease and Blau Syndrome
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Receptor Interacting Serine/Threonine Kinase 2; CARD-Containing IL-1 Beta ICE-Kinase; Receptor-Interacting Protein 2; Tyrosine-Protein Kinase RIPK2; EC 2.7.11.1; CARDIAK; RIP-2; RIP2; RICK; Receptor-Interacting Protein (RIP)-Like Interacting Caspase-Like Apoptosis Regulatory Protein (CLARP) Kinase; CARD-Containing Interleukin-1 Beta-Converting Enzyme (ICE)-Associated Kinase; CARD-Containing Interleukin-1 Beta-Converting Enzyme-Associated Kinase
- Gene ID
- 8767
- UniProt ID
- O43353
- Sequence Similarities
- Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
- Cellular Localization
- Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Polyubiquitinated via both 'Lys-63'- and 'Met-1'-linked polyubiquitin following recruitment by NOD1 or NOD2, creating docking sites for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling (PubMed:22607974, 29452636, 30026309).
'Lys-63'-linked polyubiquitination by XIAP is essential for NOD2 signaling and promotes recruitment of the LUBAC complex (PubMed:22607974, 29452636, 30026309).
Also polyubiquitinated with 'Lys-63'-linked chains by PELI3, BIRC2/c-IAP1 and BIRC3/c-IAP2 (PubMed:19464198, 21931591).
Ubiquitinated on Lys-209 via 'Lys-63'-linked by ITCH (PubMed:18079694, 19592251).
Undergoes 'Lys-63'-linked deubiquitination by MYSM1 to attenuate NOD2-mediated inflammation and tissue damage ().
Polyubiquitinated with 'Lys-63'-linked chains in response to Shigella infection, promoting its SQSTM1/p62-dependent autophagic degradation (PubMed:36221902).
Undergoes 'Met-1'-linked polyubiquitination; the head-to-tail linear polyubiquitination is mediated by the LUBAC complex in response to NOD2 stimulation 'Met-1'-linked polyubiquitination (PubMed:23806334).
'Lys-63'-linked polyubiquitination by XIAP is required for recruimtent of the LUBAC complex and subsequent (PubMed:22607974).
Linear polyubiquitination is restricted by FAM105B/otulin, probably to limit NOD2-dependent pro-inflammatory signaling activation of NF-kappa-B (PubMed:23806334).
Ubiquitination at Lys-503 by ZNRF4 via 'Lys-48'-linked polyubiquitination promotes RIPK2 degradation by the proteasome; ubiquitination by ZNRF4 takes place during both acute and NOD2 tolerance conditions (PubMed:28656966).
- Protein Refseq
- XP_011515659.1; XP_054217414.1; NP_001362289.1; NP_003812.1
- Function
- Acts as a key effector of NOD1 and NOD2 signaling pathways: upon activation by bacterial peptidoglycans, NOD1 and NOD2 oligomerize and recruit RIPK2 via CARD-CARD domains, leading to the formation of RIPK2 filaments.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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