Rabbit Anti-RNF14 Recombinant Antibody (clone R01-1H7) (CAT#: VS3-FY2080)

• Immunogen
• Synthetic peptide of human RNF14

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat RNF14

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R01-1H7

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 54 kDa, while the observed molecular weight stands at 54 kDa.

• Related Disease
• Cancer

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Ring Finger Protein 14; RING Finger Protein 14; ARA54; HFB30; Androgen Receptor Associated Protein 54; Androgen Receptor-Associated Protein 54

• Gene ID
• 9604

• UniProt ID
• Q9UBS8

• Sequence Similarities
• Belongs to the RBR family. RNF14 subfamily.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination. (Q9UBS8-RNF14_HUMAN)
  Ubiquitination at Lys372 and Lys386 (NX_Q9UBS8 [NX_Q9UBS8-1])
  Modification sites at PhosphoSitePlus (Q9UBS8)
  Glycosylation from GlyGen (Q9UBS8) 1 site, 1 O-linked glycan (1 site)

• Protein Refseq
• XP_011536016.1; XP_016865563.1; XP_016865564.1; XP_024302033.1; XP_047273854.1

• Function
• Specifically required to resolve RNA-protein cross-links caused by reactive aldehydes, which trigger translation stress by stalling ribosomes: acts by catalying 'Lys-6'-linked ubiquitination of RNA-protein cross-links, leading to their removal by the ATP-dependent unfoldase VCP and subsequent degradation by the proteasome.
  Independently of its function in the response to stalled ribosomes, acts as a regulator of transcription in Wnt signaling via its interaction with TCF transcription factors (TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4).
  May also play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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