Rabbit Anti-RNF14 Recombinant Antibody (clone R01-1H7)
CAT#: VS3-FY2080
This R01-1H7 recombinant monoclonal antibody exhibits a high affinity for human RNF14. It cross-reacts with mouse, rat. This RNF14 antibody has been rigorously validated and is widely used in western blot analysis of RNF14 in mouse brain, rat brain lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64746/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8716/22596_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8716/22596_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8716/22596_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8716/22596_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8716/22596_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8716/22596_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000013561-RNF14
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WB
Figure 1 Anti-RNF14 Antibody (VS3-FY2080) in WB.
The RNF14 expression in mouse brain, rat brain lysates were detected using the anti-RNF14 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human RNF14
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat RNF14
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-1H7
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 54 kDa, while the observed molecular weight stands at 54 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Ring Finger Protein 14; RING Finger Protein 14; ARA54; HFB30; Androgen Receptor Associated Protein 54; Androgen Receptor-Associated Protein 54
- Gene ID
- 9604
- UniProt ID
- Q9UBS8
- Sequence Similarities
- Belongs to the RBR family. RNF14 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination. (Q9UBS8-RNF14_HUMAN)
Ubiquitination at Lys372 and Lys386 (NX_Q9UBS8 [NX_Q9UBS8-1])
Modification sites at PhosphoSitePlus (Q9UBS8)
Glycosylation from GlyGen (Q9UBS8) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011536016.1; XP_016865563.1; XP_016865564.1; XP_024302033.1; XP_047273854.1
- Function
- Specifically required to resolve RNA-protein cross-links caused by reactive aldehydes, which trigger translation stress by stalling ribosomes: acts by catalying 'Lys-6'-linked ubiquitination of RNA-protein cross-links, leading to their removal by the ATP-dependent unfoldase VCP and subsequent degradation by the proteasome.
Independently of its function in the response to stalled ribosomes, acts as a regulator of transcription in Wnt signaling via its interaction with TCF transcription factors (TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4).
May also play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RNF14"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1295MZ | Recombinant Mouse Anti-Human RNF14 Antibody | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3081 | Hi-Affi™ Recombinant Rabbit Anti-RNF14 Monoclonal Antibody (DS3081AB) | WB, FC | IgG |
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