Recombinant Llama Anti-RTA Single Domain Antibody (A3C8)

Figure 1 Western analysis to identify residues important for Ab binding using mutants of RTA.

His-tagged sdAb A3C8 was used as the primary Ab. A mouse anti-His-tag Ab was used as the secondary Ab, and an anti-mouse alkaline phosphatase linked Ab was used for detection.

Legler, P. M., Compton, J. R., Hale, M. L., Anderson, G. P., Olson, M. A., Millard, C. B., & Goldman, E. R. (2017, January). Stability of isolated antibody-antigen complexes as a predictive tool for selecting toxin neutralizing antibodies. In MAbs (Vol. 9, No. 1, pp. 43-57). Taylor & Francis.