Recombinant Alpaca Anti-RTA Single Domain Antibody (PABC-569) (CAT#: PABC-569)
This product is a recombinant Alpaca antibody that can recognize RTA. V1C7 binds epitope(s) associated with weaker toxin neutralizing activity. V1C7's interface with RTA is limited to α-helix D (residues 141–142, 145–146, 149, and 153) and α-helix E (residue 163), along with several additional interactions with loops e-d (residues 65–68), C-D (residue 138), D-E (residues 157–160), and F-G (residues 195, 197).
Specific Inquiry
Figure 3 Relationships between VHH binding affinities and toxin-neutralizing activities.
Each color represents a family; each dot represents an individual VHH within that family. The downward slope of the lines suggests a relationship between KD and TNA within a clonal family.
Vance, D. J., Tremblay, J. M., Rong, Y., Angalakurthi, S. K., Volkin, D. B., Middaugh, C. R., ... & Mantis, N. J. (2017). High-resolution epitope positioning of a large collection of neutralizing and nonneutralizing single-domain antibodies on the enzymatic and binding subunits of ricin toxin. Clin. Vaccine Immunol., 24(12), e00236-17.
ELISA
Figure 4 4 Validation of cross-competition ELISA as a strategy for epitope localization on RTA.
V1C7 were subjected to competition ELISAs with a panel of RTA-specific MAbs representing neutralizing clusters I to IV.
Vance, D. J., Tremblay, J. M., Rong, Y., Angalakurthi, S. K., Volkin, D. B., Middaugh, C. R., ... & Mantis, N. J. (2017). High-resolution epitope positioning of a large collection of neutralizing and nonneutralizing single-domain antibodies on the enzymatic and binding subunits of ricin toxin. Clin. Vaccine Immunol., 24(12), e00236-17.
Specifications
- Host Species
- Alpaca
- Type
- Alpaca VHH
- Specificity
- RTA
- Species Reactivity
- Castor bean
- Clone
- PABC-569
- Applications
- ELISA, FC, Neut
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for ELISA, Flow Cytometry, Neutralization. For details, refer to Published Data.
Target
- Alternative Names
- Ricin A chain; Ricin; RTA; Ricinus communis
- UniProt ID
- P02879
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single Domain Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-567 | Recombinant Alpaca Anti-RTA Single Domain Antibody (PABC-567) | ELISA, FC, Neut | Alpaca VHH |
| PABC-568 | Recombinant Llama Anti-RTA Single Domain Antibody (PABC-568) | ELISA, WB, Neut | Llama VHH |
| PABC-570 | Recombinant Alpaca Anti-RTA Single Domain Antibody (PABC-570) | ELISA, FC, Neut | Alpaca VHH |
| PABC-571 | Recombinant Alpaca Anti-RTA Single Domain Antibody (PABC-571) | ELISA, Neut | Alpaca VHH |
| PABC-572 | Recombinant Alpaca Anti-RTA Single Domain Antibody (PABC-572) | ELISA, Neut | Alpaca VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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