Recombinant Alpaca Anti-RTA Single Domain Antibody (V1C7) (CAT#: PABC-569)

This product is a recombinant Alpaca antibody that can recognize RTA. V1C7 binds epitope(s) associated with weaker toxin neutralizing activity. V1C7's interface with RTA is limited to α-helix D (residues 141–142, 145–146, 149, and 153) and α-helix E (residue 163), along with several additional interactions with loops e-d (residues 65–68), C-D (residue 138), D-E (residues 157–160), and F-G (residues 195, 197).

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  • Size:
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  • Published Data
  • Datasheet
  • MSDS
  • COA
Neut

Figure 1 Neutralization capacity of V1C7 point mutants.

The two VHH point mutants at position 29 were compared against their parental VHHs in a Vero cell cytotoxicity assay. Ricin toxin-neutralizing activities associated with V1C7 versus V1C7 G29R.

ELISA

Figure 2 Relative affinities of V1C7 (circles), V2B9 (squares), V2E8 (triangles), and V5C1 (inverted triangles) for ricin toxin, as determined by ELISA in which ricin was captured onto plastic surface by ASF.

Bazzoli, A., Vance, D. J., Rudolph, M. J., Rong, Y., Angalakurthi, S. K., Toth IV, R. T., ... & Mantis, N. J. (2017). Using homology modeling to interrogate binding affinity in neutralization of ricin toxin by a family of single domain antibodies. Proteins: Structure, Function, and Bioinformatics, 85(11), 1994-2008.

Figure 3 Relationships between VHH binding affinities and toxin-neutralizing activities.

Each color represents a family; each dot represents an individual VHH within that family. The downward slope of the lines suggests a relationship between KD and TNA within a clonal family.

Vance, D. J., Tremblay, J. M., Rong, Y., Angalakurthi, S. K., Volkin, D. B., Middaugh, C. R., ... & Mantis, N. J. (2017). High-resolution epitope positioning of a large collection of neutralizing and nonneutralizing single-domain antibodies on the enzymatic and binding subunits of ricin toxin. Clin. Vaccine Immunol., 24(12), e00236-17.

ELISA

Figure 4 4 Validation of cross-competition ELISA as a strategy for epitope localization on RTA.

V1C7 were subjected to competition ELISAs with a panel of RTA-specific MAbs representing neutralizing clusters I to IV.

Vance, D. J., Tremblay, J. M., Rong, Y., Angalakurthi, S. K., Volkin, D. B., Middaugh, C. R., ... & Mantis, N. J. (2017). High-resolution epitope positioning of a large collection of neutralizing and nonneutralizing single-domain antibodies on the enzymatic and binding subunits of ricin toxin. Clin. Vaccine Immunol., 24(12), e00236-17.


Specifications

  • Host Species
  • Alpaca
  • Type
  • Alpaca VHH
  • Specificity
  • RTA
  • Species Reactivity
  • Castor bean
  • Clone
  • V1C7
  • Applications
  • ELISA, FC, Neut

Applications

  • Application Notes
  • The antibody was validated for ELISA, Flow Cytometry, Neutralization. For details, refer to Published Data.

Target

  • Alternative Names
  • Ricin A chain; Ricin; RTA; Ricinus communis

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "RTA"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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