Mouse Anti-SARS-COV Recombinant Antibody (clone F26G6)
CAT#: FAMAB-0673WJ
This product is a mouse monoclonal antibody F26G6 targeting SARS-COV. F26G6 was generated by immunizing with inactivated SARS-CoV. F26G6 can be used in the development of diagnostic tools and passive treatments for SARS-CoV infection.
Published Data
ELISA
Figure 1 ELISA reactivity of mAbs with whole, inactivated SARS-CoV.
Hybridoma supernatants were tested at a 1:4 dilution in PBS+0.2% BSA on pre-blocked plates, coated with 18 ng per well of inactivated, highly purified SARS-CoV. Positive clones were identified as having positive binding (color) in wells that were at least four-fold higher than the background level reactivity on BSA. Antigen legend: black bars-native, highly purified SARS-CoV; white bars-heat denatured, highly purified SARS-CoV; grey bars-BSA control.
Berry, J. D., Jones, S., Drebot, M. A., Andonov, A., Sabara, M., Yuan, X. Y., ... & Plummer, F. (2004). Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. Journal of virological methods, 120(1), 87-96.
WB
Figure 2 Western immunoblot reactivity of mAbs with highly purified SARS-CoV.
The positive and preimmune control sera were from the corresponding immune mouse and tested at 1:2000 dilution in TBS+0.2% BSA. Lanes marked 1 were loaded with highly purified SARS-CoV, lanes marked 2 with infected Vero cell lysate.
Berry, J. D., Jones, S., Drebot, M. A., Andonov, A., Sabara, M., Yuan, X. Y., ... & Plummer, F. (2004). Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. Journal of virological methods, 120(1), 87-96.
ELISA
Figure 3 Competition ELISA measuring the binding of murine mAbs to highly purified SARS-CoV in the presence of human patient serum.
Dilutions (as indicated) of a normal human serum control (white bar), or serum from convalescent SARS patient S3 were applied to wells coated with highly purified whole SARS-CoV. mAb F26G6 (Spike specific; black bars) or F26G15 (nucleoprotein specific; grey bars) were then added to the reactions.
Berry, J. D., Jones, S., Drebot, M. A., Andonov, A., Sabara, M., Yuan, X. Y., ... & Plummer, F. (2004). Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. Journal of virological methods, 120(1), 87-96.
IF
Figure 4 Immunofluorescence staining of SARS-CoV-infected Vero cells with Western immunoblot negative, neutralising mAbs to SARS-CoV.
Berry, J. D., Jones, S., Drebot, M. A., Andonov, A., Sabara, M., Yuan, X. Y., ... & Plummer, F. (2004). Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. Journal of virological methods, 120(1), 87-96.
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Specifications
- Immunogen
- Highly purified, inactivated SARS-CoV
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG2b, κ
- Clone
- F26G6
- Applications
- ELISA, WB, Neut
- Related Disease
- SARS-CoV infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Severe acute respiratory syndrome coronavirus; SARS-COV; SARS-CoV-1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1909-FY | Human Anti-SARS-CoV Recombinant Antibody (clone 03-001) | ELISA | Human IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1909-FY-F(E) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-001); scFv Fragment | ELISA | Human scFv |
| HPAB-1910-FY-F(E) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-002); scFv Fragment | ELISA | Human scFv |
| HPAB-1911-FY-F(E) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-009); scFv Fragment | ELISA | Human scFv |
| HPAB-1912-FY-F(E) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-018); scFv Fragment | ELISA | Human scFv |
| HPAB-1913-FY-F(E) | Human Anti-SARS-CoV Recombinant Antibody (clone G1); scFv Fragment | ELISA, IF, IHC | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1909-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-001); Fab Fragment | ELISA | Human Fab |
| HPAB-1910-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-002); Fab Fragment | ELISA | Human Fab |
| HPAB-1911-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-009); Fab Fragment | ELISA | Human Fab |
| HPAB-1912-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-018); Fab Fragment | ELISA | Human Fab |
| HPAB-1913-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone G1); Fab Fragment | ELISA, IF, IHC | Humanized Fab |
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