Mouse Anti-SARS-COV Recombinant Antibody (clone F26G8) (CAT#: FAMAB-0674WJ)

This product is a mouse monoclonal antibody F26G8 targeting SARS-COV. F26G8 can be used in the development of diagnostic tools and passive treatments for SARS-CoV infection.


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ELISA

Figure 1 ELISA reactivity of mAbs with whole, inactivated SARS-CoV.

Figure 1 ELISA reactivity of mAbs with whole, inactivated SARS-CoV.

Hybridoma supernatants were tested at a 1:4 dilution in PBS+0.2% BSA on pre-blocked plates, coated with 18 ng per well of inactivated, highly purified SARS-CoV. Positive clones were identified as having positive binding (color) in wells that were at least four-fold higher than the background level reactivity on BSA. Antigen legend: black bars-native, highly purified SARS-CoV; white bars-heat denatured, highly purified SARS-CoV; grey bars-BSA control.

Berry, J. D., Jones, S., Drebot, M. A., Andonov, A., Sabara, M., Yuan, X. Y., ... & Plummer, F. (2004). Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. Journal of virological methods, 120(1), 87-96.

WB

Figure 2 Western immunoblot reactivity of mAbs with highly purified SARS-CoV.

Figure 2 Western immunoblot reactivity of mAbs with highly purified SARS-CoV.

The positive and preimmune control sera were from the corresponding immune mouse and tested at 1:2000 dilution in TBS+0.2% BSA. Lanes marked 1 were loaded with highly purified SARS-CoV, lanes marked 2 with infected Vero cell lysate.

Berry, J. D., Jones, S., Drebot, M. A., Andonov, A., Sabara, M., Yuan, X. Y., ... & Plummer, F. (2004). Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. Journal of virological methods, 120(1), 87-96.


Specifications

  • Immunogen
  • Highly purified, inactivated SARS-CoV
  • Host Species
  • Mouse
  • Derivation
  • Hybridoma
  • Type
  • Mouse IgG2a, κ
  • Clone
  • F26G8
  • Applications
  • ELISA, WB, Neut
  • Related Disease
  • SARS-CoV infection

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Severe acute respiratory syndrome coronavirus; SARS-COV; SARS-CoV-1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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