Rabbit Anti-Seh1L Recombinant Antibody (clone R03-9E2) (CAT#: VS3-FY1878)
Clone R03-9E2 is a rabbit recombinant antibody that has a high affinity for human Seh1L protein, a calculated weight of 40 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R03-9E2 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Specifications
- Immunogen
- Synthetic peptide of mouse SEH1L
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat Seh1L
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-9E2
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 40 kDa, while the observed molecular weight stands at 40 kDa.
- Related Disease
- Nail Disorder, Nonsyndromic Congenital, 4 and Nonsyndromic Congenital Nail Disorder
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- SEH1 Like Nucleoporin; Nup107-160 Subcomplex Subunit SEH1; GATOR Complex Protein SEH1; Sec13 Like Protein; SEC13-Like Protein; SEC13L; Seh1
- Gene ID
- 72124
- UniProt ID
- Q8R2U0
- Sequence Similarities
- Belongs to the WD repeat SEC13 family.
- Cellular Localization
- Centromere, Chromosome, Kinetochore, Lysosome, Membrane
- Post Translation Modifications
- Ubiquitination at Lys12, Lys96, Lys306, and Lys314 (NX_Q96EE3 [NX_Q96EE3-2])
Modification sites at PhosphoSitePlus (Q96EE3)
Glycosylation from GlyGen (Q96EE3) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005258209.1; XP_011524044.1; XP_011524045.1; XP_011524046.1; XP_016881514.1
- Function
- Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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