Mouse Anti-SEMA3A Recombinant Antibody (clone 1G6F9)
CAT#: VS7-0425-WR840
This product is a mouse IgG1 antibody that binds to human SEMA3A. The antibody 1G6F9 recognizes specific amino acid domain of human SEMAPHORIN-3A (AA: 359-372). It can be used for FC assay.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52235/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human liver
Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29447/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52235/816_B11_3_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52235/790_B11_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29447/61370_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29447/61370_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29447/61370_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29447/61370_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29447/61370_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29447/61370_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (AF22, BJ, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000075213-SEMA3A
❮
❯
Specifications
- Immunogen
- Recombinant human SEMAPHORIN-3A (AA: 359-372) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SEMA3A
- Species Reactivity
- Human
- Clone
- 1G6F9
- Molecule Class
- Class 3 Semaphorins
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Hypogonadotropic hypogonadism 16 with or without anosmia (HH16)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
- Gene ID
- 10371
- UniProt ID
- Q14563
- Long Name
- Semaphorin 3A
- Cellular Localization
- Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_006071.1
- Function
- Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2258-VHH | Recombinant Anti-Human SEMA3A VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2222z | Mouse Anti-SEMA3A Recombinant Antibody (clone 28B6) | WB, ICC, IHC, IP | Mouse IgG1, κ |
| MOB-1860CT | Recombinant Mouse anti-Human SEMA3A Monoclonal antibody (EML1051) | WB | |
| HPAB-3172LY | Human Anti-SEMA3A Recombinant Antibody (HPAB-3172LY) | Neut, Inhib | Human IgG |
| HPAB-3173LY | Human Anti-SEMA3A Recombinant Antibody (HPAB-3173LY) | Neut, Inhib | Human IgG |
| HPAB-3174LY | Human Anti-SEMA3A Recombinant Antibody (HPAB-3174LY) | FuncS, Neut, Inhib | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-421CT | Humanized Anti-SEMA3A Recombinant Antibody (TAB-421CT) | WB, ELISA | Humanized IgG |
| TAB-421CT-S(P) | Humanized Anti-SEMA3A Recombinant Antibody; scFv Fragment (TAB-421CT-S(P)) | WB, ELISA | Humanized scFv |
| TAB-421CT-F(E) | Humanized Anti-SEMA3A Recombinant Antibody; Fab Fragment (TAB-421CT-F(E)) | WB, ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-078CN | Human Anti-SEMA3A Recombinant Antibody (NS-078CN) | ELISA | Chimeric (avian/human) IgG1 |
| NS-079CN | Human Anti-SEMA3A Recombinant Antibody (NS-079CN) | ELISA | Chimeric (avian/human) IgG1 |
| NS-078CN-F(E) | Human Anti-SEMA3A Recombinant Antibody; Fab Fragment (NS-078CN-F(E)) | ELISA | Chimeric (avian/human) Fab |
| NS-079CN-F(E) | Human Anti-SEMA3A Recombinant Antibody; Fab Fragment (NS-079CN-F(E)) | ELISA | Chimeric (avian/human) Fab |
| NS-078CN-S(P) | Human Anti-SEMA3A Recombinant Antibody; scFv Fragment (NS-078CN-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3172LY-S(P) | Human Anti-SEMA3A Recombinant Antibody; scFv Fragment (HPAB-3172LY-S(P)) | ELISA, Inhib | Human scfv |
| HPAB-3173LY-S(P) | Human Anti-SEMA3A Recombinant Antibody; scFv Fragment (HPAB-3173LY-S(P)) | ELISA, Inhib | Human scfv |
| HPAB-3174LY-S(P) | Human Anti-SEMA3A Recombinant Antibody; scFv Fragment (HPAB-3174LY-S(P)) | ELISA, Inhib | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3172LY-F(E) | Human Anti-SEMA3A Recombinant Antibody; Fab Fragment (HPAB-3172LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-3173LY-F(E) | Human Anti-SEMA3A Recombinant Antibody; Fab Fragment (HPAB-3173LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-3174LY-F(E) | Human Anti-SEMA3A Recombinant Antibody; Fab Fragment (HPAB-3174LY-F(E)) | ELISA, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC287 | Recombinant Anti-SEMA3A Eliminating Antibody, pH-Sensitive (VS-0625-YC287) | Antigen-Sweeping In Vivo. | Human IgG |
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