Mouse Anti-SIGLEC7 Recombinant Antibody (HPAB-2548LY)
CAT#: HPAB-2548LY
This product is a recombinant mouse antibody that recognizes SIGLEC7. The affinity constant (KD) to human Siglec-7 is 17 pM. The peptide recognized by antibody corresponds to amino acid residues 113-123, ARMSDAGRYFF. The antibody was purified by affinity chromatography. It can be used for ELISA, FC, Block, SPR, FuncS and other applications.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-SIGLEC7 Recombinant Antibody (HPAB-2548LY) in WB
Western blot analysis of HPAB-2548LY was performed with human SIGLEC7 protein.
HPAB-2548LY incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
ELISA
Figure 2 Mouse Anti-SIGLEC7 Recombinant Antibody (HPAB-2548LY) in ELISA
ELISA analysis of HPAB-2548LY was performed by coating with human SIGLEC7 protein.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
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Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75513/2007_A9_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168995-SIGLEC7
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Specifications
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SIGLEC7
- Species Reactivity
- Human
- Applications
- ELISA, FC, Block, SPR, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Incredible Affinity Data
The affinity constant of 17 pM for Human Siglec-7 reported for this antibody is accurate based on our SPR experiments. It binds incredibly tightly to the target. We also mapped the binding to the peptide residues 113-123 (ARMSDAGRYFF) successfully using this product.
Perfect For Blocking Studies
We used this recombinant mouse antibody for blocking assays and functional studies involving SIGLEC7. It performed exceptionally well, likely due to its specific recognition of the extracellular domain. It also worked great in Flow Cytometry, making it a versatile tool for our lab.
Q&As
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What is the binding affinity of this antibody to Human Siglec-7?
A: This recombinant mouse antibody exhibits a very high affinity for human Siglec-7, with an affinity constant of 17 pM. This strong binding makes it highly effective for your sensitive detection needs.
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Which peptide sequence does this antibody recognize?
A: The antibody recognizes a specific peptide corresponding to amino acid residues 113-123 of the target protein. The sequence is ARMSDAGRYFF. It is suitable for applications such as ELISA, FC, Block, SPR, and Functional Studies.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| HPAB-2547LY-F(E) | Mouse Anti-SIGLEC7 Recombinant Antibody; Fab Fragment (HPAB-2547LY-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2548LY-F(E) | Mouse Anti-SIGLEC7 Recombinant Antibody; Fab Fragment (HPAB-2548LY-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2549LY-F(E) | Mouse Anti-SIGLEC7 Recombinant Antibody; Fab Fragment (HPAB-2549LY-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2550LY-F(E) | Mouse Anti-SIGLEC7 Recombinant Antibody; Fab Fragment (HPAB-2550LY-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2551LY-F(E) | Mouse Anti-SIGLEC7 Recombinant Antibody; Fab Fragment (HPAB-2551LY-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2547LY | Mouse Anti-SIGLEC7 Recombinant Antibody (HPAB-2547LY) | ELISA, FC, Block, SPR, FuncS | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2549LY | Mouse Anti-SIGLEC7 Recombinant Antibody (HPAB-2549LY) | ELISA, FC, Block, SPR, FuncS | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2550LY | Mouse Anti-SIGLEC7 Recombinant Antibody (HPAB-2550LY) | ELISA, FC, Block, SPR, FuncS | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2551LY | Mouse Anti-SIGLEC7 Recombinant Antibody (HPAB-2551LY) | ELISA, FC, Block, SPR, FuncS | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2547LY-S(P) | Mouse Anti-SIGLEC7 Recombinant Antibody; scFv Fragment (HPAB-2547LY-S(P)) | ELISA, FC | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2548LY-S(P) | Mouse Anti-SIGLEC7 Recombinant Antibody; scFv Fragment (HPAB-2548LY-S(P)) | ELISA, FC | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2549LY-S(P) | Mouse Anti-SIGLEC7 Recombinant Antibody; scFv Fragment (HPAB-2549LY-S(P)) | ELISA, FC | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2550LY-S(P) | Mouse Anti-SIGLEC7 Recombinant Antibody; scFv Fragment (HPAB-2550LY-S(P)) | ELISA, FC | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2551LY-S(P) | Mouse Anti-SIGLEC7 Recombinant Antibody; scFv Fragment (HPAB-2551LY-S(P)) | ELISA, FC | Mouse scfv |
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| VS-0425-YC145 | Recombinant Anti-SIGLEC7 Vesicular Antibody, EV Displayed (VS-0425-YC145) | ELISA, FC, Neut, Cell-uptake |
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