Rabbit Anti-SMURF2 Recombinant Antibody (clone R06-5K4) (CAT#: VS3-XY3510)
This product is a recombinant rabbit antibody that recognizes SMURF2 (SMAD specific E3 ubiquitin protein ligase 2), which negatively regulates TGFB1-induced epithelial-mesenchymal transition. The antibody specifically recognizes human SMURF2. It also has cross-reactivity against mouse and rat SMURF2. This antibody has been reported for use in Western Blot.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/1533_A8_2_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/1533_A8_2_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_B_8_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_B_8_5.jpg
Figure 3 Stomach
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_A_5_1.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_A_5_1.jpg
Figure 4 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_A_9_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_A_9_5.jpg
Figure 5 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_A_5_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_A_5_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (BJ, HBF TERT88, LHCN-M2, TIME)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108854-SMURF2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108854-SMURF2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SMURF2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-5K4
- Applications
- WB
- Related Disease
- Breast Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- SMAD specific E3 ubiquitin protein ligase 2
- Gene ID
- 64750
- UniProt ID
- Q9HAU4
- Long Name
- SMAD specific E3 ubiquitin protein ligase 2
- Cellular Localization
- Nucleus, Cytoplasm, Cell membrane
- Post Translation Modifications
- Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
Ubiquitinated by the SCF(FBXL15) complex and TTC3, leading to its degradation by the proteasome.
'Lys-48'-linked polyubiquitination mediated by TRAF4 at Lys-119 leads to SMURF2 proteasomal degradation.
- Protein Refseq
- XP_047292502.1; XP_005257642.1
- Function
- E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SMURF2"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3347 | Hi-Affi™ Recombinant Rabbit Anti-SMURF2 Monoclonal Antibody (DS3347AB) | WB | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1412-CN | Recombinant Rabbit Anti-SMURF2 Monoclonal Antibody (JU32-34) | WB | Rabbit IgG |
| VS3-WK1161 | Mouse Anti-SMURF2 Recombinant Antibody (VS3-WK1161) | WB | Mouse IgG1, κ |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-XY3510. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.