Rabbit Anti-SMURF2 Recombinant Antibody (clone R06-5K4)
CAT#: VS3-XY3510
This product is a recombinant rabbit antibody that recognizes SMURF2 (SMAD specific E3 ubiquitin protein ligase 2), which negatively regulates TGFB1-induced epithelial-mesenchymal transition. The antibody specifically recognizes human SMURF2. It also has cross-reactivity against mouse and rat SMURF2. This antibody has been reported for use in Western Blot.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/1533_A8_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_B_8_5.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_A_5_1.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71508/159688_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ, HBF TERT88, LHCN-M2, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108854-SMURF2
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SMURF2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-5K4
- Applications
- WB
- Related Disease
- Breast Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- SMAD specific E3 ubiquitin protein ligase 2
- Gene ID
- 64750
- UniProt ID
- Q9HAU4
- Long Name
- SMAD specific E3 ubiquitin protein ligase 2
- Cellular Localization
- Nucleus, Cytoplasm, Cell membrane
- Post Translation Modifications
- Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
Ubiquitinated by the SCF(FBXL15) complex and TTC3, leading to its degradation by the proteasome.
'Lys-48'-linked polyubiquitination mediated by TRAF4 at Lys-119 leads to SMURF2 proteasomal degradation.
- Protein Refseq
- XP_047292502.1; XP_005257642.1
- Function
- E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-XY3510. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SMURF2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3347 | Hi-Affi™ Recombinant Rabbit Anti-SMURF2 Monoclonal Antibody (DS3347AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1412-CN | Recombinant Rabbit Anti-SMURF2 Monoclonal Antibody (JU32-34) | WB | Rabbit IgG |
| VS3-WK1161 | Mouse Anti-SMURF2 Recombinant Antibody (VS3-WK1161) | WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2124 | Anti-SMURF2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6762 | Anti-Mouse SMURF2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6761 | Anti-Human SMURF2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, IP, WB, IHC, IF, FuncS
Application: WB, ELISA, IF, FuncS, Apop, BA
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
