Rabbit Anti-SNAP25 Recombinant Antibody (clone R01-1C6) (CAT#: VS3-FY1882)
Clone R01-1C6 is a rabbit recombinant monoclonal antibody that exhibits high affinity to human SNAP25 protein, a calculated weight of 23 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R01-1C6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71013/1916_E10_26_cr5c99f28cf12b4_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71013/1916_E10_26_cr5c99f28cf12b4_selected.jpg
Figure 2 Cerebral cortex
(Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/360/2293_B_8_5.jpg
(Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/360/2293_B_8_5.jpg
Figure 3 RNA cell line category: Cell line enhanced (Karpas-707, SCLC-21H, SH-SY5Y, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132639-SNAP25/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132639-SNAP25/subcellular
Specifications
- Immunogen
- Synthetic peptide of human SNAP25
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SNAP25
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-1C6
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 23 kDa, while the observed molecular weight stands at 23 kDa.
- Related Disease
- Myasthenic Syndrome, Congenital, 18 and Presynaptic Congenital Myasthenic Syndromes
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Synaptosome Associated Protein 25; Resistance To Inhibitors Of Cholinesterase 4 Homolog; Synaptosomal-Associated Protein, 25kDa; SNAP-25; SNAP; SUP; Synaptosomal-Associated 25 KDa Protein; Synaptosome Associated Protein 25kDa; Synaptosomal-Associated Protein 25
- Gene ID
- 6616
- UniProt ID
- P60880
- Sequence Similarities
- Belongs to the SNAP-25 family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Synapse, Synaptosome
- Post Translation Modifications
- Palmitoylated (PubMed:28757145)., Cys-85 appears to be the main site, and palmitoylation is required for membrane association ()., (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release (PubMed:15592454, PubMed:9886085)., (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release (PubMed:9886085, PubMed:17718519)., C.botulinum type C only rarely infects humans., (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release (PubMed:9886085).
- Protein Refseq
- NP_001309831.1; NP_001309832.1; NP_001309833.1
- Function
- t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-912 | Recombinant Anti-SNAP25 Antibody | ELISA, WB, FuncS | IgG |
| MOB-0211MZ | Recombinant Mouse Anti-SNAP-25 (amino acid 183-197) cleaved Antibody (clone C318M) | ELISA, IHC | Mouse antibody |
| HPAB-0021-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0021-CN) | ELISA, ICC, IP | Mouse IgG3, κ |
| HPAB-0023-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0023-CN) | ELISA, ICC, IHC | Mouse IgG2a, κ |
| HPAB-0024-CN | Human Anti-SNAP25 Recombinant Antibody (clone h3C1A5) | ELISA, ICC, IP, IF | Humanized IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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