Rabbit Anti-SNRPA1 Recombinant Antibody (clone R69-2F-4)
CAT#: VS3-FY2144
Clone R69-2F-4 is a rabbit recombinant antibody that exhibits high affinity to human SNRPA1 protein, a calculated weight of 28 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R69-2F-4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45622/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48499/1748_E10_21_cr5805e23dd222d_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach, upper
Immunohistochemical staining of human stomach, upper shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45622/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45622/103223_A_5_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45622/103223_A_7_8.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45622/103223_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45622/103223_A_7_4.jpg
Normal Tissue
Figure 8 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45622/103223_A_2_3.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45622/103223_A_9_5.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131876-SNRPA1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SNRPA1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R69-2F-4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 28 kDa, while the observed molecular weight stands at 28kDa.
- Related Disease
- Primary Optic Atrophy and Galloway-Mowat Syndrome 1
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Small Nuclear Ribonucleoprotein Polypeptide A'; Small Nuclear Ribonucleoprotein Polypeptide A; U2 SnRNP A; U2 Small Nuclear Ribonucleoprotein Polypeptide A; U2 Small Nuclear Ribonucleoprotein A; Lea1
- Gene ID
- 6627
- UniProt ID
- P09661
- Sequence Similarities
- Belongs to the U2 small nuclear ribonucleoprotein A family.
- Cellular Localization
- Nucleus, Spliceosome
- Post Translation Modifications
- Ubiquitination at Lys149, Lys179, Lys205, and Lys221 (NX_P09661 [NX_P09661-1])
Modification sites at PhosphoSitePlus (P09661)
Glycosylation from GlyGen (P09661) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_003081.2
- Function
- Involved in pre-mRNA splicing as component of the spliceosome.
Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SNRPA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3358 | Hi-Affi™ Recombinant Rabbit Anti-SNRPA1 Monoclonal Antibody (DS3358AB) | WB, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2132 | Anti-SNRPA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6782 | Anti-Mouse SNRPA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6781 | Anti-Human SNRPA1 Immunohistochemistry Kit | IHC |
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