Rabbit Anti-STAMBP Recombinant Antibody (clone R07-6D1)
CAT#: VS3-FY1969
The antibody can be tested in western blot analysis of AMSH in A549, HL-60, U2OS lysates. This incredible rabbit recombinant monoclonal antibody that demonstrated capability to target human STAMBP. The R07-6D1 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Tested Data
Gene Expression
WB
Figure 1 Anti-STAMBP Antibody (VS3-FY1969) in WB.
The AMSH expression in A549, HL-60, U2OS lysates were detected using the anti-AMSH by western blot assay.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35800/380_E5_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35800/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35800/73383_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35800/73383_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35800/73383_A_8_5.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35800/73383_A_7_4.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35800/73383_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35800/73383_A_7_8.jpg
Normal Tissue
Figure 9 Prostate
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35800/73383_A_3_5.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enriched (LHCN-M2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124356-STAMBP/subcellular
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Specifications
- Immunogen
- Synthetic peptide of human AMSH
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human STAMBP
- Species Reactivity
- Human
- Clone
- R07-6D1
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 48 kDa, while the observed molecular weight stands at 48 kDa.
- Related Disease
- Microcephaly-Capillary Malformation Syndrome and Camptodactyly-Arthropathy-Coxa Vara-Pericarditis Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- AMSH; MICCAP
- Gene ID
- 10617
- UniProt ID
- O95630
- Sequence Similarities
- Belongs to the peptidase M67C family.
- Cellular Localization
- Cytoplasm, Endosome, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylated after BMP type I receptor activation. (O95630-STABP_HUMAN)
Ubiquitinated by SMURF2 in the presence of RNF11. (O95630-STABP_HUMAN)
Ubiquitination at Lys107, Lys153, and Lys393 (NX_O95630 [NX_O95630-1])
Modification sites at PhosphoSitePlus (O95630)
Glycosylation from GlyGen (O95630) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011530787.1; XP_047298917.1; XP_047298918.1; XP_047298919.1; XP_047298920.1
- Function
- Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF.
Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.
Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "STAMBP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3422 | Hi-Affi™ Recombinant Rabbit Anti-STAMBP Monoclonal Antibody (DS3422AB) | WB, ICC | IgG |
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