Mouse Anti-Sumo tag Recombinant Antibody (ZG-0279U)
CAT#: ZG-0279U
Mouse anti-Sumo tag antibody displayed high specificity against the Sumo tag.
Tested Data
WB
Figure 1 Mouse Anti-Sumo tag Antibody (ZG-0279U) in WB
SUMO-tagged fusion protein (20 ng/ml) was subjected to SDS-PAGE followed by with ZG-0279U at dilution of
Lane 1: 1000 Lane 4: 8000 Lane 7: 64000
Lane 2: 2000 Lane 5: 16000 Lane 8: 128000
Lane 3: 4000 Lane 6: 32000 Lane 9: 256000
Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 55kd
Observed band size: 55kd
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Specifications
- Immunogen
- sumo tag protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Sumo tag
- Species Reactivity
- All
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
- Alternative Names
- SUMO tag; SUMO
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Excellent Tag Detection
This mouse antibody is excellent for detecting Sumo tags. We used it in Western blot to verify the expression of our Sumo-tagged recombinant proteins, and it produced clean, specific bands with no background issues.
Sensitive ELISA Reagent
We employed this antibody in ELISA to quantify Sumo-tagged proteins in our lysates. The sensitivity was high, allowing us to detect even low levels of the tagged protein. It is a very reliable reagent for tag detection.
Q&As
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Is this antibody specific for the Sumo tag?
A: Yes, this Mouse Anti-Sumo tag Recombinant Antibody displays high specificity against the Sumo tag. It allows for the detection of Sumo-tagged proteins in various expression systems.
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What are the recommended dilutions for Western Blot?
A: We recommend a starting dilution of 1:500-1:5000 for Western Blot (WB). Specific conditions should be optimized by the end user. The antibody is also tested for use in Enzyme-linked Immunosorbent Assay (ELISA).
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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