Mouse Anti-TBC1D4 Recombinant Antibody (clone 8A11A2)
CAT#: VS7-0425-WR949
This product is a mouse IgG1 antibody that binds to human TBC1D4. The antibody 8A11A2 recognizes specific amino acid domain of human TBC1D4 (AA: 574-712). It can be used for FC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40145/if_selected.jpg
Normal Tissue
Figure 2 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40145/126560_A_4_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40145/126560_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40145/126560_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40145/126560_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40145/126560_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40145/126560_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (CACO-2, HUVEC TERT2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136111-TBC1D4
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Specifications
- Immunogen
- Recombinant human TBC1D4 (AA: 574-712) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TBC1D4
- Species Reactivity
- Human
- Clone
- 8A11A2
- Molecule Class
- Rab-Gtpase-Activating Protein
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Type 2 diabetes mellitus 5 (T2D5)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TBC1 Domain Family Member 4; Akt Substrate Of 160 KDa; AS160; TBC (Tre-2, BUB2, CDC16) Domain-Containing Protein; TBC1 Domain Family, Member 4; KIAA0603; NIDDM5
- Gene ID
- 9882
- UniProt ID
- O60343
- Long Name
- TBC1 Domain Family Member 4
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Acetylation; Methylation; Phosphoprotein
- Protein Refseq
- NP_001273587.1
- Function
- GTPase activation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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Reconstitution Buffer
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3508 | Rabbit Anti-TBC1D4 Recombinant Antibody (clone DS3508AB) | WB | Rabbit IgG |
| MOR-4674 | Rabbit Anti-TBC1D4 Recombinant Antibody (clone TH188DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1428 | Recombinant Rabbit Anti-TBC1D4 Antibody (clone R07-3H7) | WB, IP | Rabbit IgG |
| VS3-WK2093 | Rabbit Anti-TBC1D4 (pT642) Recombinant Antibody (clone R83-5T-8) | WB | Rabbit IgG |
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