Rabbit Anti-TCEA1 Recombinant Antibody (clone R02-6C9)
CAT#: VS3-FY1895
Clone R02-6C9 is a rabbit recombinant monoclonal antibody that is specifically engineered to target human TCEA1. It cross-reacts with mouse, rat, monkey. This TCEA1 antibody is guaranteed for western blot analysis of TCEA1 in K562, C6, 3T3 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43786/555_D9_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43786/97897_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43786/97897_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43786/97897_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43786/97897_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43786/97897_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43786/97897_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187735-TCEA1
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WB
Figure 1 Anti-TCEA1 Antibody (VS3-FY1895) in WB.
The TCEA1 expression in K562, C6, 3T3 lysates were detected using the anti-TCEA1 by western blot assay.
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Specifications
- Immunogen
- Recombinant human TCEA1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Monkey TCEA1
- Species Reactivity
- Human, Mouse, Rat, Monkey
- Clone
- R02-6C9
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 34 kDa, while the observed molecular weight stands at 34 kDa.
- Related Disease
- Cockayne Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Transcription Elongation Factor A1; Transcription Elongation Factor S-II Protein 1; Transcription Elongation Factor A (SII), 1; Transcription Elongation Factor TFIIS.O; Transcription Factor IIS; GTF2S
- Gene ID
- 6917
- UniProt ID
- P23193
- Sequence Similarities
- Belongs to the TFS-II family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys29, Lys55, Lys67, and Lys252 (NX_P23193 [NX_P23193-1])
Modification sites at PhosphoSitePlus (P23193)
Glycosylation from GlyGen (P23193) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006716530.1; XP_011515880.1; XP_054217085.1; XP_054217086.1; NP_006747.1
- Function
- Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TCEA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1907MZ | Recombinant Mouse Anti-Human TCEA1 Antibody | ELISA, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3519 | Rabbit Anti-TCEA1 Recombinant Antibody (clone DS3519AB) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2253 | Anti-TCEA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7129 | Anti-Mouse TCEA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7128 | Anti-Human TCEA1 Immunohistochemistry Kit | IHC |
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