Rabbit Anti-TRAM2 Recombinant Antibody (clone R01-7F4) (CAT#: VS3-XY3530)
The recombinant rabbit antibody reacts with TRAM2 (Translocation associated membrane protein 2), which is necessary for collagen type I synthesis. This antibody specifically reacts with human, mouse and rat TRAM2. It has been tested using Western Blot. The recommended dilutions of Western Blot is 1/500-1/1000.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35092/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35092/if_selected.jpg
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows moderate cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57925/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows moderate cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57925/ihc_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57925/142894_A_8_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57925/142894_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57925/142894_A_8_4.jpg
(Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57925/142894_A_8_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57925/142894_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57925/142894_A_8_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065308-TRAM2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065308-TRAM2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TRAM2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-7F4
- Applications
- WB
- Related Disease
- Epilepsy; Myoclonic Juvenile
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- Translocation associated membrane protein 2
- Gene ID
- 9697
- UniProt ID
- Q15035
- Long Name
- Translocation associated membrane protein 2
- Cellular Localization
- Membrane
- Post Translation Modifications
- Glycosylation at Asn55 (NX_Q15035 [NX_Q15035-1])
Modification sites at PhosphoSitePlus (Q15035)
Glycosylation from GlyGen (Q15035) 1 site
- Protein Refseq
- XP_054212856.1; XP_011513307.1
- Function
- Necessary for collagen type I synthesis.
May couple the activity of the ER Ca(2+) pump SERCA2B with the activity of the translocon.
This coupling may increase the local Ca(2+) concentration at the site of collagen synthesis, and a high Ca(2+) concentration may be necessary for the function of molecular chaperones involved in collagen folding.
Required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, may act as a ceramide sensor for regulated alternative translocation (RAT).
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
TLR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "TRAM2"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3673 | Rabbit Anti-TRAM2 Recombinant Antibody (clone DS3673AB) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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