Rabbit Anti-TRPV5 Recombinant Antibody (clone R03-7A4)
CAT#: VS3-FY1916
This R03-7A4 recombinant monoclonal antibody is specifically engineered to target human TRPV5. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. This trpV5 antibody has been rigorously validated and is widely used in western blot analysis of TRPV5 in K562, C6, 3T3, Hela lysates.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63175/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63175/151786_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63175/151786_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63175/151786_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63175/151786_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63175/151786_A_4_6.jpg
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WB
Figure 1 Anti-TRPV5 Antibody (VS3-FY1916) in WB.
The TRPV5 expression in K562, C6, 3T3, Hela lysates were detected using the anti-TrpV5 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human TRPV5
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TRPV5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-7A4
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 83 kDa.
- Related Disease
- Urolithiasis and Pseudohypoaldosteronism
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Transient Receptor Potential Cation Channel Subfamily V Member 5; Epithelial Calcium Channel 1; Calcium Transport Protein 2; Osm-9-Like TRP Channel 3; OTRPC3; ECAC1
- Gene ID
- 56302
- UniProt ID
- Q9NQA5
- Sequence Similarities
- Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV5 sub-subfamily.
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Glycosylated. (Q9NQA5-TRPV5_HUMAN)
Glycosylation at Asn358 (NX_Q9NQA5 [NX_Q9NQA5-1])
Modification sites at PhosphoSitePlus (Q9NQA5)
Glycosylation from GlyGen (Q9NQA5) 1 site
- Protein Refseq
- NP_062815.3
- Function
- Constitutively active calcium selective cation channel thought to be involved in Ca²⁺ reabsorption in kidney and intestine.
Required for normal Ca²⁺ reabsorption in the kidney distal convoluted tubules (By similarity).
The channel is activated by low internal calcium level and the current exhibits an inward rectification.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TRPV5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2037CT | Recombinant Mouse anti-Human TRPV5 Monoclonal antibody (EML1796) | ICC, IF, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3698 | Rabbit Anti-TRPV5 Recombinant Antibody (clone DS3698AB) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7561 | Anti-TRPV5 Immunohistochemistry Kit | IHC |
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