Rabbit Anti-UFC1 Recombinant Antibody (clone R08-5E1) (CAT#: VS3-FY1928)
This R08-5E1 recombinant monoclonal antibody has been meticulously crafted to specifically bind to human UFC1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The UFC1 antibody is guaranteed for western blot analysis of UFC1 in K562, rat brain, C6, 3T3 lysates and immunohistochemistry analysis of paraffin-embedded human tonsil.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Anti-UFC1 Antibody (VS3-FY1928) in WB.
The UFC1 expression in K562, rat Brain, C6, 3T3 lysates were detected using the anti-UFC1 by western blot assay.
IHC
Figure 2 Anti-UFC1 Antibody (VS3-FY1928) in IHC.
Immunohistochemistry staining of paraffin-embedded human tonsil using the anti-UFC1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Recombinant human UFC1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat UFC1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-5E1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 19 kDa, while the observed molecular weight stands at 19 kDa.
- Related Disease
- Neurodevelopmental Disorder With Spasticity And Poor Growth and Osteoarthritis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Ubiquitin-Fold Modifier Conjugating Enzyme 1; Ufm1-Conjugating Enzyme 1; Ubiquitin-Fold Modifier-Conjugating Enzyme 1; HSPC155
- Gene ID
- 51506
- UniProt ID
- Q9Y3C8
- Sequence Similarities
- Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.
- Post Translation Modifications
- Ubiquitination at Lys33 and Lys122 (NX_Q9Y3C8 [NX_Q9Y3C8-1])
Modification sites at PhosphoSitePlus (Q9Y3C8)
Glycosylation from GlyGen (Q9Y3C8) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005245311.1; XP_054192944.1; XP_054192945.1; XP_054192946.1; XP_054192947.1
- Function
- E1-like enzyme which specifically catalyzes the second step in ufmylation.
Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7731 | Anti-UFC1 Immunohistochemistry Kit | IHC |
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For Research Use Only. Not For Clinical Use.
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