Rabbit Anti-UFC1 Recombinant Antibody (clone R08-5E1) (CAT#: VS3-FY1928)
This R08-5E1 recombinant monoclonal antibody has been meticulously crafted to specifically bind to human UFC1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The UFC1 antibody is guaranteed for western blot analysis of UFC1 in K562, rat brain, C6, 3T3 lysates and immunohistochemistry analysis of paraffin-embedded human tonsil.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Anti-UFC1 Antibody (VS3-FY1928) in WB.
The UFC1 expression in K562, rat Brain, C6, 3T3 lysates were detected using the anti-UFC1 by western blot assay.
IHC
Figure 2 Anti-UFC1 Antibody (VS3-FY1928) in IHC.
Immunohistochemistry staining of paraffin-embedded human tonsil using the anti-UFC1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Recombinant human UFC1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat UFC1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-5E1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 19 kDa, while the observed molecular weight stands at 19 kDa.
- Related Disease
- Neurodevelopmental Disorder With Spasticity And Poor Growth and Osteoarthritis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Ubiquitin-Fold Modifier Conjugating Enzyme 1; Ufm1-Conjugating Enzyme 1; Ubiquitin-Fold Modifier-Conjugating Enzyme 1; HSPC155
- Gene ID
- 51506
- UniProt ID
- Q9Y3C8
- Sequence Similarities
- Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.
- Post Translation Modifications
- Ubiquitination at Lys33 and Lys122 (NX_Q9Y3C8 [NX_Q9Y3C8-1])
Modification sites at PhosphoSitePlus (Q9Y3C8)
Glycosylation from GlyGen (Q9Y3C8) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005245311.1; XP_054192944.1; XP_054192945.1; XP_054192946.1; XP_054192947.1
- Function
- E1-like enzyme which specifically catalyzes the second step in ufmylation.
Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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