Mouse Anti-UHRF1 Recombinant Antibody (VS3-CJ68)
CAT#: VS3-CJ68
This product is a mouse antibody that recognizes UHRF1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49408/if_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55446/165825_A_8_3.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55446/165825_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55446/165825_A_7_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (MOLT-4, REH)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000276043-UHRF1
❮
❯
Specifications
- Immunogen
- Recombinant fragment of human UHRF1 (N-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human UHRF1
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- ICBP90; Np95; RNF106; TDRD22; hNP95; hUHRF1; huNp95
- Gene ID
- 29128
- UniProt ID
- Q96T88
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-298 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome.
Ubiquitinated; which leads to proteasomal degradation. Autoubiquitinated; interaction with USP7 leads to deubiquitination and prevents degradation. Ubiquitination and degradation takes place during M phase, when phosphorylation at Ser-639 prevents interaction with USP7 and subsequent deubiquitination. Polyubiquitination may be stimulated by DNA damage.
- Protein Refseq
- NP_001041666.1; NP_001276979.1; NP_001276980.1
- Function
- Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF1 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-CJ68. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "UHRF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1229MZ | Recombinant Mouse Anti-Human UHRF1 Antibody | ICC, IF, IHC-P, WB | Mouse antibody |
| ZG-0495F | Mouse Anti-UHRF1 Recombinant Antibody (ZG-0495F) | WB, IF | Mouse IgG |
| ZG-0496F | Mouse Anti-UHRF1 Recombinant Antibody (ZG-0496F) | WB | Mouse IgG |
| VS7-0425-WR1087 | Mouse Anti-UHRF1 Recombinant Antibody (clone 2A8C7) | WB, IHC | Mouse IgG1 |
| VS7-0425-WR1088 | Mouse Anti-UHRF1 Recombinant Antibody (clone 1A1B1) | WB, IHC, ICC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2434 | Anti-UHRF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7739 | Anti-Mouse UHRF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7738 | Anti-Human UHRF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7740 | Anti-Rat UHRF1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
-2.jpg)
Application: IB, ELISA, FC, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: FC, IA, IF, IP, IHC, FuncS
Application: ELISA, FC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
