Mouse Anti-UHRF2 Recombinant Antibody (VS3-CJ26) (CAT#: VS3-CJ26)
This product is a mouse antibody that recognizes UHRF2.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26633/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26633/if_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26697/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26697/if_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26633/365_H10_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26633/365_H10_1_red_green.jpg
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000147854-UHRF2
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000147854-UHRF2
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000147854-UHRF2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000147854-UHRF2
Specifications
- Immunogen
- Recombinant fragment of human UHRF2 (N-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human UHRF2
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- NIRF; URF2; RNF107; TDRD23
- Gene ID
- 115426
- UniProt ID
- Q96PU4
- Cellular Localization
- Nucleus, Chromosome
- Post Translation Modifications
- May be autoubiquitinated; which may lead to proteasomal degradation.
Phosphorylated. Phosphorylation may be mediated by CDK2.
Autosumoylated.
- Protein Refseq
- NP_690856.1
- Function
- E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair (PubMed:15178429, PubMed:29506131, PubMed:27743347, PubMed:23404503).
Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them (PubMed:27129234, PubMed:24813944).
This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression (By similarity).
Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thereby inducing G1 arrest (PubMed:15178429, PubMed:15361834, PubMed:21952639).
Ubiquitinates also PCNP leading to its degradation by the proteasome (PubMed:14741369, PubMed:12176013).
Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteosomal degradation (PubMed:29923055).
Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation (PubMed:30335751).
Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation (PubMed:33848395).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "UHRF2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-640 | Human Anti-UHRF2 Recombinant Antibody (clone D626) | IP, IF, ELISA | Human IgG |
| MOB-1719MZ | Recombinant Mouse Anti-Human UHRF2 Antibody (clone 3A11) | WB | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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