Mouse Anti-UHRF2 Recombinant Antibody (VS3-CJ26) (CAT#: VS3-CJ26)

• Immunogen
• Recombinant fragment of human UHRF2 (N-terminus)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human UHRF2

• Species Reactivity
• Human

• Applications
• WB, ELISA

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, 1% BSA, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).

• Alternative Names
• NIRF; URF2; RNF107; TDRD23

• Gene ID
• 115426

• UniProt ID
• Q96PU4

• Cellular Localization
• Nucleus, Chromosome

• Post Translation Modifications
• May be autoubiquitinated; which may lead to proteasomal degradation.
  Phosphorylated. Phosphorylation may be mediated by CDK2.
  Autosumoylated.

• Protein Refseq
• NP_690856.1

• Function
• E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair (PubMed:15178429, PubMed:29506131, PubMed:27743347, PubMed:23404503).
  Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them (PubMed:27129234, PubMed:24813944).
  This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression (By similarity).
  Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thereby inducing G1 arrest (PubMed:15178429, PubMed:15361834, PubMed:21952639).
  Ubiquitinates also PCNP leading to its degradation by the proteasome (PubMed:14741369, PubMed:12176013).
  Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteosomal degradation (PubMed:29923055).
  Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation (PubMed:30335751).
  Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation (PubMed:33848395).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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