Rabbit Anti-UQCRC2 Recombinant Antibody (clone 2F6) (CAT#: ZG-0617U)
Rabbit Anti-UQCRC2 Antibody (clone 2F6) displayed high specificity against the UQCRC2 protein of Human.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Rabbit Anti-UQCRC2 Antibody (ZG-0617U) in WB
Western Blot
Positive WB detected in:HepG2 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate
All lanes: UQCRC2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Figure 2 Rabbit Anti-UQCRC2 Antibody (ZG-0617U) in IHC
IHC image of ZG-0617U diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Figure 3 Rabbit Anti-UQCRC2 Antibody (ZG-0617U) in IHC
IHC image of ZG-0617U diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human UQCRC2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human UQCRC2
- Species Reactivity
- Human
- Clone
- 2F6
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Mitochondrial Complex V Deficiency, Nuclear Type 3 and Isolated Complex Iii Deficiency.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Cytochrome b-c1 complex subunit 2; mitochondrial; Complex III subunit 2; Core protein II; Ubiquinol-cytochrome-c reductase complex core protein 2; UQCRC2
- Gene ID
- 7385
- UniProt ID
- P22695
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "UQCRC2"
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CAT | Product Name | Application | Type |
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BRD-0620MZ | Chicken Anti-UQCRC2 (ab1) Polyclonal IgY | WB | Chicken antibody |
BRD-0621MZ | Chicken Anti-UQCRC2 (ab2) Polyclonal IgY | WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
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