Rabbit Anti-UQCRC2 Recombinant Antibody (clone 2F6) (CAT#: ZG-0617U)

Rabbit Anti-UQCRC2 Antibody (clone 2F6) displayed high specificity against the UQCRC2 protein of Human.


Specific Inquiry
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  • Tested Data
  • Gene Expression
  • Datasheet
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WB

Figure 1 Rabbit Anti-UQCRC2 Antibody (ZG-0617U) in WB

Figure 1 Rabbit Anti-UQCRC2 Antibody (ZG-0617U) in WB

Western Blot
Positive WB detected in:HepG2 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate
All lanes: UQCRC2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa

IHC

Figure 2 Rabbit Anti-UQCRC2 Antibody (ZG-0617U) in IHC

Figure 2 Rabbit Anti-UQCRC2 Antibody (ZG-0617U) in IHC

IHC image of ZG-0617U diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC

Figure 3 Rabbit Anti-UQCRC2 Antibody (ZG-0617U) in IHC

Figure 3 Rabbit Anti-UQCRC2 Antibody (ZG-0617U) in IHC

IHC image of ZG-0617U diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • A synthesized peptide derived from human UQCRC2
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human UQCRC2
  • Species Reactivity
  • Human
  • Clone
  • 2F6
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Mitochondrial Complex V Deficiency, Nuclear Type 3 and Isolated Complex Iii Deficiency.

Product Property

  • Purification
  • Affinity chromatography purified
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
    WB:1:500-1:5000, IHC:1:50-1:200

Target

  • Alternative Names
  • Cytochrome b-c1 complex subunit 2; mitochondrial; Complex III subunit 2; Core protein II; Ubiquinol-cytochrome-c reductase complex core protein 2; UQCRC2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "UQCRC2"

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0620MZ Chicken Anti-UQCRC2 (ab1) Polyclonal IgY WB Chicken antibody
BRD-0621MZ Chicken Anti-UQCRC2 (ab2) Polyclonal IgY WB Chicken antibody

Recombinant Antibody

CAT Product Name Application Type
MRO-2256-CN Rabbit Anti-UQCRC2 Polyclonal Antibody (MRO-2256-CN) WB, IF, IHC, FC Rabbit IgG
VS3-XY3535 Rabbit Anti-UQCRC2 Recombinant Antibody (clone R09-7K5) WB, IHC-P, ICC, IF, IP, FC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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