Rabbit Anti-USO1 Recombinant Antibody (clone R07-4B8) (CAT#: VS3-FY1933)
This anti-USO1 antibody detected positive in western blot analysis of USO1 in 3T3 lysates. The recombinant monoclonal antibody with high affinity in binding to human and mouse USO1. It is suitable for use in a variety of assays, including WB, IP. This R07-4B8 antibody is validated for western blot analysis of USO1 in 3T3 lysates.
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WB
Figure 1 Anti-USO1 Antibody (VS3-FY1933) in WB.
The USO1 expression in 3T3 lysates were detected using the anti-USO1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human vesicle docking protein p115
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse USO1
- Species Reactivity
- Human, Mouse
- Clone
- R07-4B8
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 108 kDa, while the observed molecular weight stands at 108 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- USO1 Vesicle Transport Factor; Transcytosis Associated Protein; TAP; VDP; USO1 Homolog, Vesicle Docking Protein (Yeast); USO1 Vesicle Docking Protein Homolog (Yeast); General Vesicular Transport Factor P115; USO1 Vesicle Docking Protein Homolog
- Gene ID
- 8615
- UniProt ID
- O60763
- Sequence Similarities
- Belongs to the VDP/USO1/EDE1 family.
- Cellular Localization
- Cytoplasm, Golgi apparatus, Membrane
- Post Translation Modifications
- Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells.
Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
(O60763-USO1_HUMAN)
Ubiquitination at Lys202, Lys580, Lys705, and Lys919 (NX_O60763 [NX_O60763-1])
Modification sites at PhosphoSitePlus (O60763)
Glycosylation from GlyGen (O60763) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006714459.1; XP_006714460.1; XP_054207120.1; XP_054207121.1; NP_001276978.1
- Function
- General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2853z | Mouse Anti-USO1 Recombinant Antibody (clone 34H6) | WB, ELISA, IHC | Mouse IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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