Rabbit Anti-USO1 Recombinant Antibody (clone R07-4B8)
CAT#: VS3-FY1933
This anti-USO1 antibody detected positive in western blot analysis of USO1 in 3T3 lysates. The recombinant monoclonal antibody with high affinity in binding to human and mouse USO1. It is suitable for use in a variety of assays, including WB, IP. This R07-4B8 antibody is validated for western blot analysis of USO1 in 3T3 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38282/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38283/ihc_selected.jpg
Normal Tissue
Figure 3 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38283/141274_A_5_6.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38283/141274_B_9_5.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38283/141274_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38283/141274_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138768-USO1
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WB
Figure 1 Anti-USO1 Antibody (VS3-FY1933) in WB.
The USO1 expression in 3T3 lysates were detected using the anti-USO1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human vesicle docking protein p115
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse USO1
- Species Reactivity
- Human, Mouse
- Clone
- R07-4B8
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 108 kDa, while the observed molecular weight stands at 108 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- USO1 Vesicle Transport Factor; Transcytosis Associated Protein; TAP; VDP; USO1 Homolog, Vesicle Docking Protein (Yeast); USO1 Vesicle Docking Protein Homolog (Yeast); General Vesicular Transport Factor P115; USO1 Vesicle Docking Protein Homolog
- Gene ID
- 8615
- UniProt ID
- O60763
- Sequence Similarities
- Belongs to the VDP/USO1/EDE1 family.
- Cellular Localization
- Cytoplasm, Golgi apparatus, Membrane
- Post Translation Modifications
- Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells.
Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
(O60763-USO1_HUMAN)
Ubiquitination at Lys202, Lys580, Lys705, and Lys919 (NX_O60763 [NX_O60763-1])
Modification sites at PhosphoSitePlus (O60763)
Glycosylation from GlyGen (O60763) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006714459.1; XP_006714460.1; XP_054207120.1; XP_054207121.1; NP_001276978.1
- Function
- General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "USO1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2853z | Mouse Anti-USO1 Recombinant Antibody (clone 34H6) | WB, ELISA, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3801 | Rabbit Anti-USO1 Recombinant Antibody (clone DS3801AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2449 | Anti-USO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7772 | Anti-Mouse USO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7771 | Anti-Human USO1 Immunohistochemistry Kit | IHC |
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